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Syntaxin 4 Antibody

Affinity purified polyclonal antibody

  • WB - Syntaxin 4 Antibody AG1193-050
    Western blot anlysis of rat heart lysate:
    1. Anti-Syntaxin 4 antibody (AG1193), (1:400).
    2. Anti-Syntaxin 4 antibody, preincubated with the control peptide antigen.
  • IHC - Syntaxin 4 Antibody AG1193-050
    Expression of Syntaxin 4 in rat pancreas
    Immunohistochemical staining of rat pancreas paraffin embedded sections using Anti-Syntaxin 4 antibody (#AG1193), (1:100). Syntaxin 4 (brown staining) is expressed in endocrine cells of the Isles of Langerhans (arrow). Hematoxilin is used as the counterstain.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q08850
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 34209 Da
Homology Mouse - identical; human - 17/22 amino acidresidues identical.
Additional Information
Gene ID 81803
Other Names Syntaxin-4, Stx4, Stx4a
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide RDRTHELRQGDNISDDEDEVRV(C), corresponding to residues 2-23 of rat Syntaxin 4 (Accession Q08850). Intracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.4 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
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Syntaxin 4 is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein superfamily. The family includes 36 members in humans and is characterized by the SNARE motif, an evolutionarily conserved stretch of 60–70 amino acids that are arranged in heptad repeats1,2. SNARE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion1,2. SNAREs can be roughly divided into vesicular (v-SNAREs) and target (t-SNAREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cognate v-/t-SNAREs between two opposing membranes generates trans-SNARE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion. Syntaxin 4, like most SNAREs, is a type IV membrane protein with a relatively large N-terminus containing the SNARE motif located in the cytoplasmic side and a transmembrane domain located close to the C-terminus that functions as an anchor1,2. Syntaxin 4 functions as a plasma membrane protein t-SNARE and has been shown to have an important role in pancreatic b-cell insulin secretion and in insulin-stimulated glucose uptake in skeletal muscle and adipose tissue by means of translocation of the glucose transporter GLUT4 from an intracellular compartment to the plasma membrane3,4. Abgent is pleased to offer a highly specific antibody directed against an epitope located in the intracellular N-terminus domain of rat Syntaxin 4. Anti-Syntaxin 4 antibody (#AG1193) can be used in western blot and immunohistochemical applications, and was designed to recognize Syntaxin 4 from rat and mouse samples.


References 1. Jahn, R. and Scheller, R.H. (2006) Nat. Rev. Mol. Cell Biol. 7, 631. 2. Südhof, T.C. and Rothman, J.E. (2009) Science 323, 474. 3. Spurlin, B.A. and Thurmond, D.C. (2006) Mol. Endocrinol. 20, 183. 4. Jewell, J.L. et al. (2010) Am. J. Physiol. Regul. Integr. Comp. Physiol. 298, R517.

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$ 475.00
$ 575.00
Cat# AG1193-050
(40 western blots)
Availability: 2-3 weeks
Bulk Size
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