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Synaptotagmin-I Antibody

Affinity purified polyclonal antibody

  • WB - Synaptotagmin-I Antibody AG1194-025
    Western blot analysis of rat brain membranes:
    1. Anti-Synaptotagmin-I antibody (#AG1194), (1:200).
    2. Anti-Synaptotagmin-I antibody, preincubated with the control peptide antigen.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P21107
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 32994 Da
Homology Mouse - 18/19 amino acid residues identical; human, bovine- 17/20 amino acid residues identical; chicken - 15/19amino acidresidues identical.
Additional Information
Gene ID 25716
Other Names Tropomyosin alpha-3 chain, Gamma-tropomyosin, Tropomyosin-3, Tpm3, Tpm-5, Tpm5
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide MVSASHPEALAAPVTTVAT(C), corresponding to amino acid residues 1-19 of rat synaptotagmin I (Accession P21107). Intravesicular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.2 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Synaptotagmin 1 is a member of the Ca2+-binding synaptotagmin family of proteins that includes 16 members in vertebrates and is involved in regulated exocytosis and trafficking1,2. Synaptotagmins are type I membrane proteins located in intracellular vesicles and consist of a short N-terminal domain located in the vesicle lumen, a transmembrane domain and a cytosolic domain containing two Ca2+ binding domains1,2. Synaptotagmins are usually localized to distinct secretory vesicles and control their Ca2+-dependent fusion. Synaptotagmin I is predominantly expressed in neuronal cells and is an abundant constituent of synaptic vesicles1,2. Synaptotagmin I has a relatively low Ca2+ affinity and is able to bind Ca2+ mostly in the vicinity of Ca2+ channels pores. Indeed, Synaptotagmin I has been demonstrated to directly interact with selected Ca2+ channels. Synaptotagmin I also binds to the t-SNARE protein Syntaxin 1 in a Ca2+-dependent manner thus forming the prototypical Ca2+-dependent fast synaptic vesicle exocytosis protein complex that also includes SNAP-25 and VAMP1,2,3. Abgent is pleased to offer a highly specific antibody directed against the intravesicular N-terminus domain of rat synaptotagmin I. Anti-Synaptotagmin I antibody (#AG1194) can be used in Western blot and immunohistochemical applications, and was designed to recognize Synaptotagmin I from rat, mouse and human samples.


References 1. Südhof, T.C. (2002) J. Biol. Chem. 277, 7629. 2. Malsam, J. et al. (2008) Cell. Mol. Life Sci. 65, 2814. 3. Meir, A. et al. (1999) Physiol. Rev.79, 1019.

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$ 375.00
$ 475.00
$ 575.00
Cat# AG1194-025
(40 western blots)
Availability: 2-3 weeks
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