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>   home   >   Products   >   Primary Antibodies   >   Nicotinic Acetylcholine Receptor beta4 (extracellular) Antibody   

Nicotinic Acetylcholine Receptor beta4 (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - Nicotinic Acetylcholine Receptor beta4 (extracellular) Antibody AG1197-050
    Western blot analysis of SH-SY5Y human neuroblastoma cell lysate (lanes 1 and 4), rat dorsal root ganglion (lanes 2 and 5) and mouse brain (lanes 3 and 6) lysates: 1-3. Anti-Nicotinic Acetylcholine Receptor β4 (extracellular) antibody (#AG1197), (1:200).
    4-6. Anti-Nicotinic Acetylcholine Receptor β4 (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - Nicotinic Acetylcholine Receptor beta4 (extracellular) Antibody AG1197-050
    Expression of Nicotinic Acetylcholine Receptor β4 in rat dorsal root ganglion (DRG)

    Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-Nicotinic Acetylcholine Receptor β4 (extracellular) antibody (#AG1197). A. AChRβ4 labeling (red) appears in neuronal cell bodies. B. Nuclear staining using DAPI as counter stain (blue). C. Merged images of A+B.

  • ICC - Nicotinic Acetylcholine Receptor beta4 (extracellular) Antibody AG1197-050
    Expression of Nicotinic Acetylcholine Receptor β4 in rat PC12 cells Immunocytochemical staining of live intact rat pheochromocytoma PC12 cells. A. Cells were stained with Anti-Nicotinic Acetylcholine Receptor β4 (extracellular) antibody (#AG1197), (1:100), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Cell nuclei were visualized using Hoechst 33342 (blue). C. Merge of the two images.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P12392
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 55861 Da
Homology Mouse - identical; human - 13/14 amino acid residues identical.
Additional Information
Gene ID 25103
Other Names Neuronal acetylcholine receptor subunit beta-4, Neuronal acetylcholine receptor non-alpha-2 chain, N-alpha 2, Chrnb4, Acrb4
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide CYEGVNILRIPAKR, corresponding to amino acid residues 95-108 of rat nAChRb4 (Accession P12392). Extracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
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Acetylcholine, released by cholinergic neurons, activates two groups of acetylcholine receptors (AChRs); muscarinic AChRs (mAChRs) which belong to the superfamily of G-protein coupled receptors (GPCRs) and nicotinic AChRs (nAChRs) which belong to the ligand-gated ion channel superfamily. nAChRs also respond to nicotine, hence their name1. To date, 17 different but related subunits of nAChRs have been identified and cloned. They consist of a subunits (α1-10), which are responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding2. The other subunits responsible for making up the active receptor are the β (β1-4), γ, δ and ε subunits3. Structurally, all subunits have the following: a conserved large extracellular N-terminal domain, 3 conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-terminal domain.  An active nAChR is generally a heteropentamer of these various subunits organized around a central pore1. While most β subunits are neuronal, the β1 subunit forms functional receptors along with other subunits in the muscle3. In neurons the α2-α6 and β2-β4 subunits form heteropentameric receptors, usually with a (αx)2y)3 stoichiometry3. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat Nicotinic Acetylcholine Receptor β4 (nAChRβ4). Anti-Nicotinic Acetylcholine Receptor β4 (extracellular) antibody (#AG1197) can be used in western blot analysis and immunocytochemistry applications, and has been designed to recognize nAChRβ4 from mouse, rat and human samples.


References 1. Albuquerque, E.X. et al. (2009) Physiol. Rev. 89, 73. 2. Karlin, A. et al. (1986) Ann. NY. Acad. Sci. 463, 53. 3. Kalamida, D. et al. (2007) FEBS J. 274, 3799.

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$ 475.00
$ 575.00
Cat# AG1197-050
(40 western blots)
Availability: 2-3 weeks
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