Nicotinic Acetylcholine Receptor beta2 (extracellular) Antibody
Affinity purified polyclonal antibody
|Application ||WB, LCI|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||56909 Da|
|Homology||Mouse - identical; human - 11/12 amino acid residues identical.|
|Other Names||Neuronal acetylcholine receptor subunit beta-2, Neuronal acetylcholine receptor non-alpha-1 chain, N-alpha 1, Chrnb2, Acrb2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)EDFDNMKKVRLP, corresponding to amino acid residues 96-107 of rat nAChR־²2 (Accession P12390). Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Acetylcholine, released by cholinergic neurons, activates two groups of acetylcholine receptors (AChRs); muscarinic AChRs (mAChRs) which belong to the superfamily of G-protein coupled receptors (GPCRs) and nicotinic AChRs (nAChRs) which belong to the ligand-gated ion channel superfamily. nAChRs also respond to nicotine, hence their name1. To date, 17 different but related subunits of nAChRs have been identified and cloned. They consist of α subunits (α1-10), which is responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding2. The other subunits responsible for making up the active receptor are the β (β1-4), γ, δ and ε subunits3. Structurally, all subunits have the following: a conserved large extracellular N-terminal domain, 3 conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-terminal domain. An active nAChR is generally a heteropentamer of these various subunits organized around a central pore1. While most β subunits are neuronal, the β1 subunit forms functional receptors along with other subunits in the muscle3. The diversity of these receptors and their functional organization gives rise to unique properties and functions. The α4β2 receptor composition makes up a high affinity nicotinic receptor. In fact, its upregulation (mainly expressed by the increase of functional receptors at the membrane and not expression per se) is responsible for the increased appearance of binding sites following nicotine administration1,3. Animal studies have shown that nAChR-related mechanisms are involved in attention function4. Indeed α4β2 nAChR seems to also be involved in attention-deficit hyperactivity disorder (ADHD), a disease distinguished by a lack of attention, distractibility and hyperactivity3. The α4β2 and α7 nAChRs appear to be critical in rats for attention and working memory. Also, a α4β2 specific agonist was shown to reduce impulsivity, hyperactivity and attention deficits in adults with ADHD5. This same receptor subtype may also be involved in Parkinson’s disease (PD) as smoking and α4β2 nAChR agonists show beneficial effects in PD6,7. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope rat Nicotinic Acetylcholine Receptor β2 (nAChRβ2). Anti-Nicotinic Acetylcholine Receptor β2 (extracellular) antibody (#AG1198) can be used in western blot and immunocytochemistry applications, and has been designed to recognize nAChRβ2 from mouse, rat and human samples.
References 1. Albuquerque, E.X. et al. (2009) Physiol. Rev. 89, 73. 2. Karlin, A. et al. (1986) Ann. NY. Acad. Sci. 463, 53. 3. Kalamida, D. et al. (2007) FEBS J. 274, 3799. 4. Blonde, A. et al. (2000) Psychopharmacology (Berlin) 149, 293. 5. Wilens, T.E. et al. (1999) Am. J. Psychiatry 156, 1931. 6. Tanner, C.M. et al. (2002) Neurology 58, 581. 7. Schneider, J.S. et al. (1998) Ann. Neurol. 43, 311.
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