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>   home   >   Products   >   Primary Antibodies   >   Nicotinic Acetylcholine Receptor Alpha6 (extracellular) Antibody   

Nicotinic Acetylcholine Receptor Alpha6 (extracellular) Antibody

Affinity purified polyclonal antibody

     
  • WB - Nicotinic Acetylcholine Receptor Alpha6 (extracellular) Antibody AG1199-025
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: 1,2. Anti-Nicotinic Acetylcholine Receptor α6 (extracellular) antibody (#AG1199), (1:1000).
    3,4. Anti-Nicotinic Acetylcholine Receptor α6 (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - Nicotinic Acetylcholine Receptor Alpha6 (extracellular) Antibody AG1199-025
    Expression of nAChRα6 in rat striatum Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Nicotinic Acetylcholine Receptor α6 (extracellular) antibody (#AG1199), (1:100). A. nAChRα6 staining (red) is detected in the striatal matrix (M) but not in the striatal patches (arrows) nor in the overlying corpus callosum (CC). B. DAPI (blue) counterstain reveals the outline of the striatal matrix and patches and the overlying corpus callosum.
  • ICC - Nicotinic Acetylcholine Receptor Alpha6 (extracellular) Antibody AG1199-025
    Expression of nAChRα6 in rat PC12 cells Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-Nicotinic Acetylcholine Receptor α6 (extracellular) antibody (#AG1199), (1:50), followed by goat anti-rabbit-AlexaFluor- 594 secondary antibody (red). B. Live image of the cells. C. Merge of the two images.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, LCI
Primary Accession P43143
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 56947 Da
Homology Mouse - 12/13 amino acid residues identical.
Additional Information
Gene ID 81721
Other Names Neuronal acetylcholine receptor subunit alpha-6, Chrna6, Acra6
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)EQLFHTLFAHYNR, corresponding to amino acid residues 35-47 of rat nAChR־±6 (Accession P43143). Extracellular, N-terminus.Not recommended for human samples.
Dilution WB~~1:200-1:2000
IHC~~1:100
ICC~~1:50
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000ֲ x g 5 min).
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 3 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Citations (0)

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Background

Neuronal nicotinic acetylcholine receptors (nAChRs) belong to the superfamily of ligand-gated ion channels and are widely expressed throughout the central and peripheral nervous systems. nAChRs play crucial roles in modulating a wide range of higher cognitive functions by mediating presynaptic, postsynaptic, and extrasynaptic signaling1.   Nicotinic acetylcholine receptors containing α6 subunits (nAChRα6) exhibit a unique expression pattern in the mammalian brain. nAChRs α6 are abundantly expressed in the midbrain dopaminergic (DAergic) system, including mesocorticolimbic and nigrostriatal pathways, and particularly present in presynaptic nerve terminals2. nAChRα6 expressed on DAergic neurons can be activated by endogenous acetylcholine (Ach) or exogenous nicotine and analogs, which suggests that the activation of nAChRα6 may play a vital role in central cholinergic circuits, including modulation of locomotor behaviour and drug addiction3. In addition, nAChRα6 is particularly susceptible to nigrostriatal damage, which may lead to Parkinson’s disease (PD)4. nAChRα6 are also expressed by sensory neurons; therefore, they may also play a role in disorders related to sensory perception5.

References

References 1. Betz, H. (1990) Neuron 5, 383. 2. Champtiaux, N. et al. (2003) J Neurosci. 23, 7820. 3. le Novere, N. et al. (1999) Neuroreport 10, 2497. 4. Quik, M. et al. (2007) Biochem. Pharmacol. 74, 1224. 5. Hone, A.J. et al. (2012) FASEB J. 26, 917.

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Cat# AG1199-025
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