Nicotinic Acetylcholine Receptor Alpha6 (extracellular) Antibody
Affinity purified polyclonal antibody
|Application ||WB, LCI|
|Calculated MW||56947 Da|
|Homology||Mouse - 12/13 amino acid residues identical.|
|Other Names||Neuronal acetylcholine receptor subunit alpha-6, Chrna6, Acra6|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)EQLFHTLFAHYNR, corresponding to amino acid residues 35-47 of rat nAChR־±6 (Accession P43143). Extracellular, N-terminus.Not recommended for human samples.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000ֲ x g 5 min).|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||3 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
Neuronal nicotinic acetylcholine receptors (nAChRs) belong to the superfamily of ligand-gated ion channels and are widely expressed throughout the central and peripheral nervous systems. nAChRs play crucial roles in modulating a wide range of higher cognitive functions by mediating presynaptic, postsynaptic, and extrasynaptic signaling1. Nicotinic acetylcholine receptors containing α6 subunits (nAChRα6) exhibit a unique expression pattern in the mammalian brain. nAChRs α6 are abundantly expressed in the midbrain dopaminergic (DAergic) system, including mesocorticolimbic and nigrostriatal pathways, and particularly present in presynaptic nerve terminals2. nAChRα6 expressed on DAergic neurons can be activated by endogenous acetylcholine (Ach) or exogenous nicotine and analogs, which suggests that the activation of nAChRα6 may play a vital role in central cholinergic circuits, including modulation of locomotor behaviour and drug addiction3. In addition, nAChRα6 is particularly susceptible to nigrostriatal damage, which may lead to Parkinson’s disease (PD)4. nAChRα6 are also expressed by sensory neurons; therefore, they may also play a role in disorders related to sensory perception5.
References 1. Betz, H. (1990) Neuron 5, 383. 2. Champtiaux, N. et al. (2003) J Neurosci. 23, 7820. 3. le Novere, N. et al. (1999) Neuroreport 10, 2497. 4. Quik, M. et al. (2007) Biochem. Pharmacol. 74, 1224. 5. Hone, A.J. et al. (2012) FASEB J. 26, 917.
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