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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Serotonin Transporter (SERT) (extracellular) Antibody   

Serotonin Transporter (SERT) (extracellular) Antibody

Affinity purified polyclonal antibody

     
  • WB - Serotonin Transporter (SERT) (extracellular) Antibody AG1204-025
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: 1-2.  Anti-Serotonin Transporter (SERT) (extracellular) antibody (#AG1204), (1:200).
    3-4.  Anti-Serotonin Transporter (SERT) (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - Serotonin Transporter (SERT) (extracellular) Antibody AG1204-025
    Expression of SERT in mouse brain Immunohistochemical staining of immersion-fixed, free floating mouse brain frozen sections using Anti-Serotonin Transporter (SERT) (extracellular) antibody (#AG1204), (1:400). A. SERT (green) was visualized in axonal fibers of the hippocampal dentate gyrus. B. Axonal fibers of neurons expressing gamma amino butyric acid (GABA) were labeled with mouse anti parvalbumin (red). C. Merge of the two images demonstrates separate axonal processes (no co-localization).
  • IHC - Serotonin Transporter (SERT) (extracellular) Antibody AG1204-025
    Expression of SERT in rat brain Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Serotonin Transporter (SERT) (extracellular) antibody (#AG1204), (1:400). A. SERT (green) was visualized in axonal fibers of the rat hippocampal CA1 region. B. Axonal fibers of neurons expressing gamma amino butyric acid (GABA) were labeled with mouse anti parvalbumin (red). C. Merge of the two images demonstrates separate axonal processes (no co-localization).
  • ICC - Serotonin Transporter (SERT) (extracellular) Antibody AG1204-025
    Expression of Serotonin Transporter (SERT) rat PC12 cells Immunocytochemical staining of live intact rat pheochromocytoma PC12 cells. A. Cells were stained with Anti-Serotonin Transporter (SERT) (extracellular) antibody (#AG1204), (1:100), followed by goat anti-rabbit-AlexaFluor- 594 secondary antibody (red). B. Cell nuclei were visualized using Hoechst 33342 (blue). C. Live view of the cells.
  • FC - Serotonin Transporter (SERT) (extracellular) Antibody AG1204-025
    Indirect flow cytometry analysis in live intact human T cell leukemia (Jurkat) cell line: ___ Unstained cells + goat-anti-rabbit-Cy5.
    ___ Cells + Anti-Serotonin Transporter (SERT) (extracellular) antibody (#AG1204), (1:25) + goat-anti-rabbit-Cy5.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P31652
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 70172 Da
Homology Mouse, human - identical.
Additional Information
Gene ID 25553
Other Names Sodium-dependent serotonin transporter, 5HT transporter, 5HTT, Solute carrier family 6 member 4, Slc6a4
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide ֲ (C)EMRNEDVSEVAKD, corresponding to amino acids residues 388-400 of rat Serotonin Transporter (Accession P31652).ֲ ֲ 4th extracellular loop.
Dilution WB~~1:200-1:2000
IHC~~1:400
ICC~~1:100
FC~~1:25
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 ml water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Background

Many physiological, endocrine and behavioral functions are determined and regulated by monoamine signaling1,2. Many brain disorders such as depression, drug abuse, schizophrenia, attention deficit hyperactivity disorder (ADHD) are caused by the malfunction of monoaminergic transmission1-3.  The intensity of monoaminergic signaling is determined by the availability of the monoamine, which is in turn determined in part by its uptake from the extracellular milieu via monoamine transporters. These transporters include DAT, SERT, and NET, responsible for uptaking dopamine, serotonin (5-HT) and noradrenaline respectively, and recycling them back for release3-5. DAT, SERT and NET are members of the Na+/Cl- dependent membrane transporter family which also includes other members. These transporters consist of 12 transmembrane domains and intracellular N- and C-termini. Like its counterparts, SERT’s intracellular N- and C-terminal domains are also subject to phosphorylation and protein-protein interactions important for modulating its activity and localization6. SERTs are evidently expressed in serotonergic neurons, and are also expressed in various peripheral tissues including specialized cells of the gut, placenta, lung, blood lymphocytes and platelets6. SERT knockout mice are viable and as a consequence exhibit increased extracellular levels of 5-HT and significantly decreased levels of brain tissue 5-HT due to deficient recycling and re-accumulation of serotonin by SERT7,8. Phenotypes displayed by SERT knockout mice include stress sensitivity, obesity and various behavioral changes9 Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat serotonin transporter. Anti-Serotonin Transporter (SERT) (extracellular) antibody (#AG1204) can be used in western blot analysis, immunohistochemistry, immunocytochemistry and indirect flow cytometry applications and has been designed to recognize SERT from human, mouse and rat samples.

References

References 1. Carlsson, A. (1987) Annu. Rev. Neurosci. 10, 19. 2. Greengard, P. (2001) Science 294, 1024. 3. Gainetdinov, R.R. and Caron, M.G. (2003) Annu. Rev. Pharmacol. Toxicol. 43, 261. 4. Amara, S.G. and Kuhar, M.J. (1993) Trends Pharmacol. Sci. 14, 43. 5. Miller, G.W. et al. (1999) Trends Pharmacol. Sci. 20, 424. 6. Ramamoorthy, S. et al. (2010) Pharmacol. Ther. Doi. 10.1016. 7. Mathews, T.A. et al. (2004) J. Neurosci. Meth. 140, 169. 8. Kim, D.K. et al. (2005) Neuropharmacology 49, 798. 9. Haenisch, B. and Bönisch, H. (2011) Pharmacol. Ther. 129, 352.

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Cat# AG1204-025
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