Melanin-Concentrating Hormone Receptor 1 (extracellular) Antibody
Affinity purified polyclonal antibody
|Application ||WB, LCI|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||39728 Da|
|Homology||Mouse- 16/17 amino acid residues identical; human- 13/17 amino acid residues identical.|
|Other Names||Melanin-concentrating hormone receptor 1, MCH receptor 1, MCH-R1, MCHR-1, G-protein coupled receptor 24, MCH-1R, MCH1R, MCHR, SLC-1, Somatostatin receptor-like protein, Mchr1, Gpr24, Slc1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide CMDLQTSLLSTGPNASN, corresponding to amino acid residues 7-20 of rat Melanin-concentrating hormone receptor 1 (Accession P97639 ). Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis and mass-spectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Melanin-concentrating hormone (MCH) is a 19 amino acid peptide originally isolated from the salmon pituitary gland (in fish the length of the peptide is 17 amino acids)1. In rodents and humans the peptide is synthesized in the brain and is involved in a number of physiological effects, although it is best known for its central role in the control of appetite control. For example, central administration of MCH in mice leads to obesity and conversely, knockout of the MCH gene in mice yields lean animals with an increased metabolic rate and reduced body fat2,3. MCH exerts its effects by binding MCHR1 and MCHR2, two receptors belonging to the superfamily of G-protein coupled receptors (GPCRs). Like all members of the family, MCHR1 and MCHR2 have seven transmembrane domains, an extracellular N-terminal and intracellular C-terminal tails. MCHR1 and MCHR2 activation both lead to an increase in intracellular Ca2+ concentration3. In rat, MCHR1 expression is quite broad, and is strongly detected in the brain. Expression of the receptor is also seen in the eye, skeletal muscle, tongue, pituitary, intestine and lymphocytes4,5. MCHR1 is also expressed in adipose tissue where it regulates leptin secretion3. In human, MCHR1 is mostly localized to the brain and pituitary. MCHR2 displays similar localization to that of MCHR1 in the brain5,6. As MCHR2 is a pseudogene in rodents, studying its function is quite challenging. In the brain, MCHR1 is localized in many areas that are known to be involved in various psychological disorders. Indeed, MCHR1-/- animals display antianxiety- and antidepressant-like behavior7,8. Therefore, antagonizing the activity of MCHR1 could indeed serve as a means to treat depression and obesity9. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat Melanin-Concentrating Hormone Receptor 1. Anti-Melanin-Concentrating Hormone Receptor 1 (extracellular) antibody (#AG1207) can be used in western blot analysis and immunocytochemistry applications, and has been designed to recognize MCHR1 from rat, mouse and human samples.
References 1. Kawauchi, H. et al. (1983) Nature 305, 321. 2. Shimada, M. et al. (1998) Nature 396, 243. 3. Chung, S. et al. (2009) Peptides 30, 1985. 4. Saito, Y. et al. (1999) Nature 400, 265. 5. Hill, J. et al. (2001) J. Biol. Chem. 276, 20125. 6. An, S. et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 7576. 7. Roy, M. et al. (2006) Neuropsychopharmacology 31, 112. 8. Smith, D.G. et al. (2006) Neuropsychopharmacology 31, 1135. 9. Gehlert, D.R. et al. (2009) J. Pharmacol. Exp. Ther. 329, 429.
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