|Calculated MW||36953 Da|
|Homology||Rat - 12/13 amino acid residues identical; human - 7/13 amino acid residues identical.|
|Other Names||Melanocortin receptor 5, MC5-R, Mc5r|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)RYNSVRQRTSMKG,ֲ corresponding to amino acid residues 224-236 of mouse Melanocortin Receptor 5 (Accession P41149). 3rdֲ intracellular loop.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Application Details||Western blot analysis (WB): - Rat kidney and testis (see Si, J. et al. (2013) in Product Citations). Immunohistochemistry (IH): - Rat kidney sections (see Si, J. et al. (2013) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.6 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Melanocortin Receptor 5 (MC5R) is one of five members of the melanocortin receptor family, which belongs to the 7-transmembrane domain, G-Protein Coupled Receptor (GPCR) superfamily. The ligands of these receptors, the melanocortins, are a group of structurally-related peptides comprising the α-, β-, and γ-melanocyte-stimulating hormone (α-, β-, γ-MSH) and the adrenocorticotropic hormone (ACTH), all of which are derived from post-translational processing of a common precursor peptide, proopiomelanocortin (POMC) 1,2,3. One of the salient features of the melanocortin signaling system is the existence of two endogenous antagonists, proteins that bind specifically to the receptor and have an inhibitory effect. These antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP) 1. All five melanocortin receptors bind their agonists (the melanocortins) and their endogenous antagonists (agouti/ASP and AGRP) with differing affinities. The order of potency for MC5R activation is α-MSH > ACTH = β-MSH >> γ-MSH while AGRP works as an endogenous antagonists of MC5R1,2,3. MC5R is mostly expressed in peripheral tissues such as skeletal muscle, bone marrow, skin tissues particularly in sebaceous glands and exocrine glands such as salivary glands1. The best understood function of Melanocortin Receptor 5 is in the regulation of exocrine glands since MC5R-deficient mice exhibit severely defective water repulsion and thermoregulation due to decreased production of sebaceous lipids4.
References 1. Abdel-Malek, Z.A. (2001) Cell. Mol. Life Sci. 58, 434. 2. Gantz, I. and Fong, T.M. (2003) Am. J. Physiol. 284, E468. 3. Wikberg, J.E. et al. (2000) Pharmacol. Res. 42, 393. 4. Chen, W. et al. (1997) Cell 91, 789.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.