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Melanocortin Receptor 4 (extracellular) Antibody

Affinity purified rabbit polyclonal antibody

     
  • WB - Melanocortin Receptor 4 (extracellular) Antibody AG1211-025
    Western blot analysis of rat brain lysates: 1. Anti-Melanocortin Receptor 4 (extracellular) antibody (#AG1211), (1:400). 2. Anti-Melanocortin Receptor 4 (extracellular) antibody, preincubated with the control peptide antigen.
  • WB - Melanocortin Receptor 4 (extracellular) Antibody AG1211-025
    Western blot analysis of mouse brain lysates: 1. Anti-Melanocortin Receptor 4 (extracellular) antibody (#AG1211), (1:400). 2. Anti-Melanocortin Receptor 4 (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - Melanocortin Receptor 4 (extracellular) Antibody AG1211-025
    Expression of MC4R in mouse brain Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-Melanocortin Receptor 4 (extracellular) antibody (#AG1211), (1:100). MC4R (green fluorescence) is expressed in the mouse hypothalamus in axonal processes (arrows). Hoechst 33342 is used as the counterstain (blue).
  • ICC - Melanocortin Receptor 4 (extracellular) Antibody AG1211-025
    Expression of MC4R in rat pituitary cell line Immunocytochemical staining of live intact GH3 cells with Anti-Melanocortin Receptor 4 (extracellular) antibody (#AG1211), (1:50). A. MC4R staining was observed, followed by Alexa-555-conjugated goat anti-rabbit secondary antibody (red staining). Hoechst 33342 (blue) is used to visualize the nuclei. B. Live view of the same field as A.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC
Primary Accession P32245
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 36943 Da
Homology Rat, mouse - 13/15 amino acid residues identical.
Additional Information
Gene ID 4160
Other Names Melanocortin receptor 4, MC4-R, MC4R
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)HRGMHTSLHLWNRSS, corresponding to amino acid residues 6-20 of human MC4R (Accession P32245 ). Extracellular, N terminal.
Dilution WB~~1:200-1:2000
IHC~~1:100
ICC~~1:50
Peptide Confirmation Confirmed by mass-spectrography analysis.
Application Details Immunocytochemistry (IC): - Mouse hippocampal neurons (see Shen, Y. et al. (2013) in Product Citations).
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Research Areas
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Background

Melanocortin Receptor 4 (MC4R) is one of five members of the melanocortin receptor family, which belongs to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. The ligands of these receptors, the melanocortins, are a group of structurally-related peptides comprising the α-, β-, and γ-melanocyte-stimulating hormone (α-, β-, γ-MSH) and the adrenocorticotropic hormone (ACTH), all of which are derived from post-translational processing of a common precursor peptide, proopiomelanocortin (POMC).1,2,3 One of the salient features of the melanocortin signaling system is the existence of two endogenous antagonists, proteins that bind specifically to the receptor and have an inhibitory effect. These antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP).1 All five melanocortin receptors bind their agonists (the melanocortins) and their endogenous antagonists (agouti/ASP and AGRP) with differing affinities. The order of potency for MC4R activation is α-MSH = ACTH > β-MSH >> γ-MSH. AGRP and agouti/ASP are both endogenous antagonists of MC4R.1,2,3 MC4R is widely expressed in the brain including the cortex, thalamus, hypothalamus, and brain stem. The best understood physiological role of MC4R is in the regulation of feeding behavior and the control of body weight. Indeed, mice with a targeted disruption of the MC4R gene display an obese phenotype characterized by increased adiposity, hyperphagia, and hyperleptinaemia.4 Similarly, MC4R mutations in humans have been associated with severe childhood obesity including characteristics very similar to the MC4R knockout mouse phenotype.5 All these make MC4R an attractive drug target for the treatment of obesity.

References

1. Abdel-Malek, Z.A. (2001) Cell. Mol. Life Sci. 58, 434.
2. Gantz, I. and Fong, T.M. (2003) Am. J. Physiol. 284, E468.
3. Wikberg, J.E. et al. (2000) Pharmacol. Res. 42, 393.
4. Huszar, D. et al (1997) Cell 88, 131.
5. Adan, R.A. et al. (2006) Br. J. Pharmacol. 149, 815.

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Cat# AG1211-025
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