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Melanocortin Receptor 3 (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - Melanocortin Receptor 3 (extracellular) Antibody AG1212-050
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    1, 2. Anti-Melanocortin Receptor 3 (extracellular) antibody (#AG1212), (1:200).
    3, 4. Anti-Melanocortin Receptor 3 (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - Melanocortin Receptor 3 (extracellular) Antibody AG1212-050
     Expression of MC3R in rat brain Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Melanocortin Receptor 3 (extracellular) antibody (#AG1212), (1:100). Expression (green fluorescence) appears in the hypothalamic periventricular region (horizontal arrow), in some nerve cells (vertical arrow), and in the neuropil (asterisk). Hoechst 33342  is used as the counterstain (blue).
  • FC - Melanocortin Receptor 3 (extracellular) Antibody AG1212-050
    Indirect flow cytometry analysis in human promyelocytic leukemia HL-60 cells.
    ___ Cells + FITC-conjugated goat anti-rabbit antibody.
    ___ Cells + Anti-Melanocortin Receptor 3 (extracellular) antibody (#AG1212), (1:40) + FITC conjugated goat anti-rabbit antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P32244
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 35757 Da
Homology Mouse- identical; human 13/16 amino acid residues identical.
Additional Information
Gene ID 29310
Other Names Melanocortin receptor 3, MC3-R, Mc3r
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)NSDSLTLEDQFIQHMD, corresponding to amino acid residues 102-117 ofֲ rat MC3R (Accession P32244). 1st extracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Melanocortin Receptor 3 (MC3R) is one of five members of the melanocortin receptor family, which belongs to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.   The ligands of these receptors, the melanocortins, are a group of structurally-related peptides comprising the α-, ß-, and γ-melanocyte-stimulating hormone (α-, ß-, γ-MSH) and the adrenocorticotropic hormone (ACTH), all of which are derived from post-translational processing of a common precursor peptide, proopiomelanocortin (POMC).1,2,3   One of the salient features of the melanocortin signaling system is the presence of two endogenous antagonists, proteins that bind specifically to the receptor and have an inhibitory effect. These antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP).1   All five melanocortin receptors bind their agonists (the melanocortins) and their endogenous antagonists (agouti and AGRP) with differing affinities. MC3R is the only one of the melanocortin receptors that shows no difference in binding specificity for any of the melanocortins, binding all of them with equal efficiency. AGRP is the high affinity endogenous antagonist of MC3R.1,2,3   The physiological function of MC3R is still poorly understood. The distribution of MC3R in hypothalamic nuclei of the central nervous system (CNS) suggests a role in the regulation of energy homeostasis. Indeed, studies with MC3R knockout (MC3R KO) mice showed that these mice have increased body fat due to increased feed efficiency.4 In humans, several polymorphisms of the MC3R gene are associated with high insulin levels and obesity in children.5  In addition, expression of MC3R in macrophages is thought to mediate the well known anti-inflammatory effects of α-MSH.6   Abgent is pleased to offer a highly specific antibody directed against an epitope in the extracellular first loop of rat MC3R. Anti-Melanocortin Receptor 3 (extracellular) antibody (#AG1212) can be used in Western blot, immunohistochemical, and flow cytometry applications, and recognizes MC3R from human, mouse, and rat samples.


References 1. Abdel-Malek, Z.A. (2001) Cell. Mol. Life Sci. 58, 434. 2. Gantz, I. and Fong, T.M. (2003) Am. J. Physiol. Endocrinol. Metab. 284, E468. 3. Wikberg, J.E.S. et al. (2000) Pharm. Res.42, 393. 4. Butler, A.A. et al. (2000) Endocrinology 141, 3518. 5. Feng, N. et al. (2005) Diabetes 54, 2663. 6. Catania, A. et al. (2004) Pharmacol. Rev. 56, 1.

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$ 475.00
$ 575.00
Cat# AG1212-050
(40 western blots)
Availability: 2-3 weeks
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