|Application ||WB, IHC|
|Calculated MW||33927 Da|
|Homology||Rat, mouse - identical.|
|Other Names||Adrenocorticotropic hormone receptor, ACTH receptor, ACTH-R, Adrenocorticotropin receptor, Melanocortin receptor 2, MC2-R, MC2R, ACTHR|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)RNMGYLKPRGSFE, corresponding to amino acid residues 87-99 of human MC2R (Accession Q01718). 1st extracellular loop.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Melanocortin Receptor 2 (MC2R) belongs to a five-member receptor family known as the melanocortin receptors. The melanocortin receptors are members of the 7 transmembrane domain, G protein-coupled receptor (GPCR) superfamily. The receptors ligands, the melanocortins, are a group of structurally derived peptides consisting of α-, ß- and γ melanocyte stimulating hormone (α, ß, γ-MSH) and the adrenocorticotropic hormone (ACTH) all of which are derived from the post-translational processing of a common precursor peptide, proopiomelanocortin (POMC).1,2,3 One of the most salient features of the melanocortin signaling system is the presence of two endogenous antagonists, that is proteins that bind specifically to the receptor but instead of activating it have an inhibitory effect. The antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP).4 All five melanocortin receptors bind their agonists (the melanocortins) and their endogenous antagonists (agouti and AGRP) with different affinities. MC2R can be also described as the ACTH receptor as it is exclusively activated by the ACTH hormone. In fact, MC2R mediates all the physiological effects of ACTH in the adrenal cortex, that is the regulation and production of steroids, their circadian variation and their stress-related fluctuations.1, 2, 3 In addition, mutations that result in MC2R functional impairment are associated with familial glucocorticoid deficiency.4 The receptor transduces signals via Gs resulting in the activation of adenylate cyclase and production of cAMP. Abgent is pleased to offer a highly specific antibody directed against the extracellular 1st loop of the human MC2R. Anti-Melanocortin Receptor 2 (extracellular) antibody (#AG1213) can be used in Western blot and immunohistochemistry applications, and will recognize human, mouse and rat samples.
1. Abdel-Malek, Z.A. (2001) Cell. Mol. Life Sci. 58, 434.
2. Gantz, I. and Fong, T.M. (2003) Am. J. Physiol. Endocrinol. Metab. 284, E468.
3. Wikberg, J.E.S. et al. (2000) Pharm. Res.42, 393.
4. Elias, L.L. et al. (1999) J Clin Endocrinol Metab 84, 2766.
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