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Melanocortin Receptor 1 Antibody

Affinity purified polyclonal antibody

  • WB - Melanocortin Receptor 1 Antibody AG1214-050
    Western blot analysis of human normal skin fibroblast cell line Malme-3 (lanes 1 and 4) and human malignant melanoma cell lines Malme-3M (lanes 2 and 5) and A875 (lanes 3 and 6):
    1, 2, 3. Anti-Melanocortin Receptor 1 antibody (#AG1214), (1:500).
    4, 5, 6. Anti-Melanocortin Receptor 1 antibody, preincubated with the control peptide antigen
  • WB - Melanocortin Receptor 1 Antibody AG1214-050
    Western blot analysis of rat adrenal lysate: 1. Anti-Melanocortin Receptor 1 antibody (#AG1214), (1:400).
    2. Anti-Melanocortin Receptor 1 antibody, preincubated with the control peptide antigen.
  • IHC - Melanocortin Receptor 1 Antibody AG1214-050
    Expression of MC1R in normal skin and melanoma Immunohistochemical staining of paraffin embedded normal skin and melanoma sections using Anti-Melanocortin Receptor 1 antibody (#AG1214) (1:100). MCR1 staining (red-brown color is highly specific in A. epidermal cells, B. eccrine sweat gland cells and C. melanoma cells.  Color reaction was obtained with DAB. Hematoxilin is used as the counterstain.
  • ICC - Melanocortin Receptor 1 Antibody AG1214-050
    Expression of MC1R in human Malme-3M cells Immunocytochemical staining of human paraformaldehyde fixed and permeabilized malignant melanoma cell lines (Malme-3M). A. Cells were stained with Anti-Melanocortin Receptor 1 antibody (#AG1214), (1:200) followed by goat-anti-rabbit- AlexaFluor-555 secondary antibody. B. Live view of the same field as in (A).
    C. Computer merge of (A) and (B).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q01726
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 34706 Da
Homology Rat- 11/6 amino acids residues identical.
Additional Information
Gene ID 4157
Other Names Melanocyte-stimulating hormone receptor, MSH-R, Melanocortin receptor 1, MC1-R, MC1R, MSHR
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)HAQGIARLHKRQRPVH, corresponding to amino acid residues 217-232 of human MC1R (Accession Q01726). 3rd intracellular loop.Probably wonג€™t recognize mouse.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Application Details Western blot analysis (WB): - Rat kidney and testis (see Si, J. et al. (2013) in Product Citations). Immunohistochemistry (IH): - Rat kidney sections (see Si, J. et al. (2013) in Product Citations). Immunocytochemistry (IC): - Rat renal tubular epithelial cells (see Si, J. et al. (2013) in Product Citations).  
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.6 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Research Areas
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Melanocortin Receptor 1 (MC1R) belongs to a five-member receptor family known as the melanocortin receptors. The melanocortin receptors are members of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. The receptors ligands, the melanocortins, are a group of structurally derived peptides consisting of α-, β- and γ-melanocyte stimulating hormone (α, β, γ-MSH) and the adrenocorticotropic hormone (ACTH) all of which are derived from the post-translational processing of a common precursor peptide, proopiomelanocortin (POMC).1,2,3   One of the most salient features of the melanocortin signaling system is the presence of two endogenous antagonists, that is proteins that bind specifically to the receptor but instead of activating it have an inhibitory effect. The antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP).4   All five melanocortin receptors bind their agonists (the melanocortins) and their endogenous antagonists (agouti and AGRP) with different affinities.   MC1R was the first member of the melanocortin receptor family to be cloned. The receptor transduces signals via Gs resulting in the activation of adenylate cyclase and production of cAMP.    MC1R binds its ligands with the following potency: α-MSH = ACTH > β-MSH > γ-MSH. MC1R also binds the endogenous antagonist agouti with high affinity.1, 2   MC1R can be described as the “classical” melanocyte α-MSH receptor. The receptor is expressed in the skin where it has a key role in determining skin and hair pigmentation. In fur-bearing mammals, the local ratio between α-MSH and agouti will determine coat pigmentation as α-MSH stimulates and agouti inhibits melanin production.   In humans, especially in Caucasians, the MC1R gene is highly polymorphic and several allelic variants have been correlated with red-hair, poor tanning ability and increased risk of melanoma.1,3,5 In addition, MC1R expression in melanoma has been shown to be upregulated up to 20-fold when compared to normal melanocytes.   The melanocortins and particularly α-MSH have significant anti-inflammatory properties. Since α-MSH binds to MC1R with the highest potency, it was proposed that the latter mediated the anti-inflammatory effects of α-MSH. Indeed, MC1R expression has been demonstrated in several cells of the immune system including macrophages and neutrophils.6


References 1. Abdel-Malek, Z.A. (2001) Cell. Mol. Life Sci. 58, 434. 2. Gantz, I. and Fong, T.M. (2003) Am. J. Physiol. 284, E468. 3. Wikberg, J.E. et al. (2000) Pharm. Res. 42, 393. 4. Dinulescu, D.M. and Cone, R.D. (2000) J. Biol. Chem. 275, 6695. 5. Kanetsky, P.A. et al.  (2006) Cancer Res. 66, 9330. 6. Catania, A. et al. (2004) Pharmacol. Rev. 56, 1.

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Cat# AG1214-050
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