|Reactivity||Human, Mouse, Rat|
|Calculated MW||51421 Da|
|Homology||Mouse, rat - identical.|
|Other Names||Muscarinic acetylcholine receptor M1, CHRM1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)RKIPKRPGSVHRTPSR, corresponding to amino acid residues 443-458 of human M1 Muscarinic Receptor (Accession P11229). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
The action of the neurotransmitter acetylcholine is mediated through two types of receptors, the ionotrophic nicotinic receptors and the metabotrophic muscarinic receptors. The muscarinic receptors belong to the superfamily of 7-TM G-protein coupled receptors. Five subtypes of muscarinic receptors have been cloned and named m1-m51,2. The muscarinic receptors are widely distributed throughout the body, but are predominantly expressed within the parasympathetic nervous system and exert both excitatory and inhibitory control over central and peripheral tissues1,2. Muscarinic receptors participate in a number of physiological functions such as regulation of heart rate, muscle contraction, cognition, sensory processing and motor control1. They also participate in learning and memory processing3,4. The m1 receptors are the most abundant muscarinic subtype in the cortex and striatum. m1 receptors are also localized in the myenteric plexus where they function as autoreceptors to enhance the release of Ach from the nerves5,6. The m1, m3 and m5 receptors, which are coupled to Gq/11 proteins, can protect cells from apoptosis induced by DNA damage. The signaling mechanism that mediates this anti-apoptotic response is still poorly understood. However, it was recently reported that a poly-basic motif in the C-terminus tail of the m1, m3 and m5 receptors is an essential element for the anti-apoptotic response of those receptors7. Abgent is pleased to offer a highly specific antibody directed against an epitope human m1 receptor. Anti-M1 Muscarinic Receptor (443-458) antibody (#AG1215) can be used in western blot analysis and immunohistochemistry applications. It has been designed to recognize m1 from mouse, rat and human samples.
References 1. Felder, C.C. et al. (2000) J. Med. Chem. 43, 4333. 2. Forsythe, S.M. et al. (2002) Am. J. Respir. Cell. Mol. Biol. 26, 298. 3. Ferreira, A.R. et al. (2003) Pharmacol. Biochem. Behav. 74, 411. 4. Van der Zee, E.A. et al. (1999) Prog. Neurol. 58, 409. 5. Levey, A.I. et al. (1991) J. Neurosci. 11, 3218. 6. Wang, J. et al. (2000) Am. J. Physiol. 279, G1059. 7. Budd, D.C. et al. (2003) J. Biol. Chem. 278, 19565.
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