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M1 Muscarinic Receptor (443-458) Antibody

Affinity purified polyclonal antibody

  • WB - M1 Muscarinic Receptor (443-458) Antibody AG1215-050
    Western blot analysis of rat brain lysate: 1. Anti-M1 Muscarinic Receptor (443-458) antibody (#AG1215), (1:200).
    2. Anti-M1 Muscarinic Receptor (443-458) antibody, preincubated with the control antigen.
  • IHC - M1 Muscarinic Receptor (443-458) Antibody AG1215-050
    Expression of M1 receptor in mouse striatum Immunohistochemical staining of mouse striatum using Anti-M1 Muscarinic Receptor (443-458) antibody (#AG1215), (1:100). M1 Muscarinic Receptor staining (red) appears in the matrix (M), not in the patches (P). Blood vessels in the matrix (arrow) are also stained. DAPI is used as the counterstain (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P11229
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 51421 Da
Homology Mouse, rat - identical.
Additional Information
Gene ID 1128
Other Names Muscarinic acetylcholine receptor M1, CHRM1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)RKIPKRPGSVHRTPSR, corresponding to amino acid residues 443-458 of human M1 Muscarinic Receptor (Accession P11229). Intracellular, C-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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The action of the neurotransmitter acetylcholine is mediated through two types of receptors, the ionotrophic nicotinic receptors and the metabotrophic muscarinic receptors. The muscarinic receptors belong to the superfamily of 7-TM G-protein coupled receptors. Five subtypes of muscarinic receptors have been cloned and named m1-m51,2. The muscarinic receptors are widely distributed throughout the body, but are predominantly expressed within the parasympathetic nervous system and exert both excitatory and inhibitory control over central and peripheral tissues1,2. Muscarinic receptors participate in a number of physiological functions such as regulation of heart rate, muscle contraction, cognition, sensory processing and motor control1. They also participate in learning and memory processing3,4. The m1 receptors are the most abundant muscarinic subtype in the cortex and striatum. m1 receptors are also localized in the myenteric plexus where they function as autoreceptors to enhance the release of Ach from the nerves5,6. The m1, m3 and m5 receptors, which are coupled to Gq/11 proteins, can protect cells from apoptosis induced by DNA damage. The signaling mechanism that mediates this anti-apoptotic response is still poorly understood. However, it was recently reported that a poly-basic motif in the C-terminus tail of the m1, m3 and m5 receptors is an essential element for the anti-apoptotic response of those receptors7. Abgent is pleased to offer a highly specific antibody directed against an epitope human m1 receptor. Anti-M1 Muscarinic Receptor (443-458) antibody (#AG1215) can be used in western blot analysis and immunohistochemistry applications. It has been designed to recognize m1 from mouse, rat and human samples.


References 1. Felder, C.C. et al. (2000) J. Med. Chem. 43, 4333. 2. Forsythe, S.M. et al. (2002) Am. J. Respir. Cell. Mol. Biol. 26, 298. 3. Ferreira, A.R. et al. (2003) Pharmacol. Biochem. Behav. 74, 411. 4. Van der Zee, E.A. et al. (1999) Prog. Neurol. 58, 409. 5. Levey, A.I. et al. (1991) J. Neurosci. 11, 3218. 6. Wang, J. et al. (2000) Am. J. Physiol. 279, G1059. 7. Budd, D.C. et al. (2003) J. Biol. Chem. 278, 19565.

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$ 367.00
$ 475.00
Cat# AG1215-050
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