|Application ||WB, IHC, IP, ICC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||66066 Da|
|Homology||Human, mouse, bovine, pig - identical.|
|Other Names||Muscarinic acetylcholine receptor M3, Chrm3, Chrm-3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)TLAKRFALKTRSQITKRKR, corresponding to amino residues 461-479 of rat m3 (Accession number P08483). 3rd intracellular loop.|
|Peptide Confirmation||Confirmed by amino acid analysis and massspectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
The action of the neurotransmitter acetylcholine is mediated through two types of receptors, the ionotrophic nicotinic receptors and the metabotrophic muscarinic receptors. The muscarinic receptors belong to the superfamily of 7-TM G-protein-coupled receptors. Five subtypes of muscarinic receptors have been cloned and named m1-m5.1-2 The muscarinic receptors are widely distributed throughout the body, but are predominantly expressed within the parasympathetic nervous system and exerts both excitatory and inhibitory control over central and peripheral tissues.1-2 The m3 receptor is expressed in brain, heart, urinary bladder, salivary gland and airway smooth muscle.3 Muscarinic receptors participate in a number of physiologic functions such as regulation of heart rate, muscle contraction, cognition, sensory processing and motor control.1 They also participate in learning and memory processing.4-5 The m1 receptors are the most abundant muscarinic subtype in the cortex and striatum. m1 receptors were also localized in the myenteric plexus where they function as autoreceptors to enhance the release of Ach from the nerves.6-7 m2, found in heart, is coupled to and mediates the slowing of heart rate in response to acetylcholine.3,8 m3 controls smooth muscle contraction and its stimulation causes secretion from glandular tissues.9 Involvement of muscarinic receptors in apoptosis and cell growth has been reported.10-14 m1, m3 and m5, which are coupled to Gq/11 proteins, can protect cells from undergoing apoptosis induced by DNA damage. The signaling mechanism that mediates this anti-apoptotic response is still poorly understood. However, it was recently reported that a poly-basic motif in the C-terminus tail of the m3, m1 and m5 receptors is an essential element for the anti-apoptotic response of those receptors.10 Muscarinic receptors are also capable of promoting cell proliferation in many cell types, however, the signaling pathways are yet, unclear.11-14 In the literature, m3 was reported to be involved in mitogenesis and proliferation of different cell types. Recently, it was reported that activation of the m3 muscarinic receptor induces significant proliferation of colon and prostate cancer cells.11-14 Abgent is pleased to offer a highly specific antibody directed against an intracellular epitope of rat m3 muscarinic receptor. Anti-M3 Muscarinic Receptor antibody (#AG1216) can be used for Western blot analysis, immunohistochemistry, immunocytochemistry and immunoprecipitation. It has been designed to recognize m3 muscarinic receptor human, mouse and rat samples .
1. Felder, C.C. et al. (2000) J. Med. Chem. 43, 4333.
2. Forsythe, S.M. et al. (2002) Am. J. Respir. Cell. Mol. Biol. 26, 298.
3. Hulme, E. et al. (1990) Ann. Rev. Pharmac. Toxicol. 30, 633.
4. Ferreira, A.R. et al. (2003) Pharmacol. Biochem. Behav. 74, 411.
5. Van der Zee, E.A. et al. (1999) Prog. Neurol. 58, 409.
6. Levey, A.I. et al. (1991) J. Neurosci. 11, 3218.
7. Wang, J. et al. (2000) Am. J. Physiol. Gastrointest. Liver Physiol. 279, G1059.
8. Billington, C.K. and Penn, R.B. (2002) Am. J. Respir. Cell. Mol. Biol. 26, 269.
9. Ehlert, F.J. et al. (2003) Life. Sci. 74, 355.
10. Budd, S.C. et al. (2003) J. Biol. Chem. 278, 19565.
11. Frucht, H. et al. (1999) Clin. Cancer. Res. 5, 2532.
12. Rayford, W. et al. (1997) Prostate 30, 160.
13. Ukegawa, J.I. et al. (2003) J. Cancer. Res. Clin. Oncol. 129, 272.
14. Shafer, S.H. and Williams, C.L. (2004) Mol. Pharmacol. 65, 1080.
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