|Application ||WB, IHC, IP, ICC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||51715 Da|
|Homology||Chimpanzee, gorilla - identical; orangutan - 130/132 amino acid residues identical; pig - 122/132 amino acid residues identical; rat - 118/132 amino acid residues identical; mouse - 117/132 amino acid residues identical; dog - 122/132 amino acid residues identical.|
|Other Names||Muscarinic acetylcholine receptor M2, CHRM2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||GST fusion protein with a sequence VANQDPVSPSLVQGRIVKPN NNNMPSSDDGLEHNKIQNGKAPRDPVTENCVQGEEKESSNDSTSV SAVASNMRDDEITQDENTVSTSLGHSKDENSKQTCIRIGTKTPKS DSCTPTNTTVEVVGSSGQNGDE, corresponding to amino acid residues 225-356 of human m2 (Accession P08172). 3rd intracellular loop.|
|Peptide Confirmation||Confirmed by DNA sequence and SDS-PAGE.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl PBS.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||3 µg fusion protein per 1 µg antibody.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
The action of the neurotransmitter acetylcholine is mediated through two types of receptors, the ionotrophic nicotinic receptors and the metabotrophic muscarinic receptors. The muscarinic receptors belong to the superfamily of 7-TM G-protein-coupled receptors. Five subtypes of muscarinic receptors have been cloned and are named m1-m5.1-2 The muscarinic receptors are widely distributed throughout the body, but are predominantly expressed within the parasympathetic nervous system and exerts both excitatory and inhibitory control over central and peripheral tissues.1-2 Muscarinic receptors participate in a number of physiological functions such as regulation of heart rate, muscle contraction, cognition, sensory processing and motor control.1 They also participate in learning and memory processing.3-4 The m2 receptor is considered to be the predominant muscarinic receptor that is expressed in cardiac muscle.5 The m2 and m4 receptors mediate Ca2+ channel inhibition and Kir3 K+ channel activation by directly binding the Gbg subunit to the channel.6,7 Stimulation of the m2 receptor by acetylcholine in the heart results in activation of the Kir3.1/Kir3.4 channels causing a slowing in heart beat.7 Abgent is pleased to offer a highly specific antibody directed against the 3rd intracellular loop of the human m2 receptor. Anti-M2 Muscarinic Receptor antibody (#AG1218) can be used in western blot analysis, immunoprecipitation, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize m1 from mouse and rat and human samples.
1. Felder, C.C. et al. (2000) J. Med. Chem. 43, 4333.
2. Forsythe, S.M. et al. (2002) Am. J. Respir. Cell. Mol. Biol. 26, 298.
3. Ferreira, A.R. et al. (2003) Pharmacol. Biochem. Behav. 74, 411.
4. Van der Zee, E.A. and Luiten, P.G. (1999) Prog. Neurol. 58, 409.
5. Tata, A.M. et al. (1999) Brain Res. 824, 63.
6. Shapiro, M.S. et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96, 10899.
7. Jin, W. and Lu, Z. (1998) Biochemistry 37, 13291.
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