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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   GPCR Antibodies   >   Human CysLTR2 (extracellular) Antibody   

Human CysLTR2 (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - Human CysLTR2 (extracellular)  Antibody AG1221-050
    Western blot analysis of acute monocytic leukemia THP-1 (lanes 1 and 4), promyelocytic leukemia HL-60 (lanes 2 and 5) and chronic myelogenous leukemia K562 (lanes 3 and 6) human cell lysates: 1- 3. Anti-Human CysLTR2 (extracellular) antibody (#AG1221), (1:200).
    4- 6. Anti-Human CysLTR2 (extracellular) antibody, preincubated with the control peptide antigen.
  • FC - Human CysLTR2 (extracellular)  Antibody AG1221-050
    Indirect flow cytometry analysis in live intact acute monocytic leukemia (THP-1) cells: ___  Control cells + goat anti-rabbit-AlexaFluor-488.
    ___  Cells + Anti-Human CysLTR2 (extracellular) antibody (#AG1221), (1:50) + goat anti-rabbit-AlexaFluor-488.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NS75
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 39635 Da
Homology Human only.
Additional Information
Gene ID 57105
Other Names Cysteinyl leukotriene receptor 2, CysLTR2, G-protein coupled receptor GPCR21, hGPCR21, G-protein coupled receptor HG57, HPN321, CYSLTR2, CYSLT2, CYSLT2R
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)HLTTWKVGL(S)KDRLH, corresponding to amino acid residues 270-284 of human CysLTR2 (Accession Q9NS75). 3rd extracellular loop.Not recommended for use with rat and mouse samples.
Dilution WB~~1:200-1:2000
Format Affinity purified antibody, lyophilized powder
Reconstitution Add 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Research Areas
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LTC4, LTD4, and LTE4 are cysteinyl leukotrienes (cys-LTs) peptide conjugated lipids derived from arachidonic acid as a result of immune or inflammatory stimuli1-3. The above mentioned cys-LTs bind and activate two receptors belonging to the G-protein coupled receptor superfamily, CysLTR1 and CysLTR2 (also named cysLT1 and cysLT2 respectively). There is evidence though that other receptors are yet to be identified4. These two receptors couple to both pertussis toxin sensitive (Gi/o) and insensitive (Gq, which initiates Ca2+ influx) G-proteins for their signaling. The G-protein coupled to is probably determined by which ligand binds the receptor as all three cys-LTs can activate the two receptors albeit with different affinities5. In mouse, CysLTR1 and CysLTR2 are subject to alternative splicing. CysLTR1 receptor expresses greater affinity for LTD4 and binds equally to LTC4 and LTE4whereas CysLTR2 has greater and equal affinity towards LTC4 and LTD4 over LTE45. Human CysLTR1 is highly expressed in spleen, peripheral blood, leukocytes. It is less strongly expressed in lung, small intestine, pancreas and hardly or not expressed in liver, colon, kidney, skeletal muscle, testis heart and brain4. On the other hand, CysLTR2 is highly expressed in spleen and leukocytes. Its expression in the heart, brain, spinal cord and adrenal gland differs it from that of CysLTR14. Cys-LTs and their receptors are involved in respiratory diseases like asthma and other conditions such as cancer cardiovascular, gastrointestinal, skin and immune disorders1,6. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of human CysLTR2. Anti-CysLTR2 (extracellular) antibody (#AG1221) can be used for western blot analysis and indirect flow cytometry applications. It has been designed to recognize CysLTR2 from human samples only.


References 1. Rovati, G.E. and Capra, V. (2007) ScientificWorldJournal 7, 1375. 2. Samuelson, B. (1983) Science 220, 568. 3. Funk, C.D. (2001) Science 294, 1871. 4. Singh, R.K. et al. (2010) Pharmacology 85, 336. 5. Kanaoka, Y. and Boyce, J.A. (2004) J. Immunol. 173, 1503. 6. Capra, V. et al. (2006) Med. Res. Rev. 27, 469.

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$ 475.00
$ 575.00
Cat# AG1221-050
(40 western blots)
Availability: 2-3 weeks
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