|Calculated MW||38541 Da|
|Homology||Human - 14/15; mouse - 12/15 amino acid residues identical; rat - 11/15 amino acid residues identical.|
|Other Names||Cysteinyl leukotriene receptor 1, CysLTR1, Cysteinyl leukotriene D4 receptor, LTD4 receptor, G-protein coupled receptor HG55, HMTMF81, CYSLTR1, CYSLT1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)HNETKP(S)DSVLRMQK, corresponding to amino acid residues 261-275 of human CysLTR1 (Accession Q9Y271). 3rd extracellular loop.http://www.uniprot.org/uniprot/Q9Y271|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||Add 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
LTC4, LTD4, and LTE4 are leukotrienes (cys-LTs) derived from arachidonic acid as a result of immune or inflammatory stimuli1-3. The above mentioned cys-LTs bind and activate two receptors belonging to the G-protein coupled receptor superfamily, CysLTR1 and CysLTR2 (also named cysLT1 and cysLT2 respectively). There is evidence though that other receptors are yet to be identified4. These two receptors couple to both pertussis toxin sensitive (Gi/o) and insensitive (Gq, which initiates Ca2+ influx) G-proteins for their signaling. The G-protein coupled to is probably determined by which ligand binds the receptor as all three cys-LTs can activate the two receptors albeit with different affinities5. In mouse, CysLTR1 and CysLTR2 are subject to alternative splicing. CysLTR1 receptor expresses greater affinity for LTD4 and binds equally to LTC4 and LTE4 whereas CysLTR2 has greater and equal affinity towards LTC4 and LTD4 over LTE45. Human CysLTR1 is highly expressed in spleen, peripheral blood, leukocytes. It is less strongly expressed in lung, small intestine, pancreas and hardly or not expressed in liver, colon, kidney, skeletal muscle, testis heart and brain4. On the other hand, CysLTR2 is highly expressed in spleen and leukocytes. Its expression in the heart, brain, spinal cord and adrenal gland differs it from that of CysLTR14. Cys-LTs and their receptors are involved in respiratory diseases like asthma and other conditions such as cancer cardiovascular, gastrointestinal, skin and immune disorders1,6. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of human CysLTR1. Anti-CysLTR1 (extracellular) antibody (#AG1222) can be used for Western blot anlaysis and indirect flow cytometry applications. It has been designed to recognize CysLTR1 from human, mouse and rat samples.
References 1. Rovati, G.E. and Capra, V. (2007) ScientificWorldJournal 7, 1375. 2. Samuelson, B. (1983) Science 220, 568. 3. Funk, C.D. (2001) Science 294, 1871. 4. Singh, R.K. et al. (2010) Pharmacology 85, 336. 5. Kanaoka, Y. and Boyce, J.A. (2004) J. Immunol. 173, 1503. 6. Capra, V. et al. (2006) Med. Res. Rev. 27, 469.
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