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Human BLT1 (extracellular) Antibody

Affinity purified polyclonal antibody

     
  • WB - Human BLT1 (extracellular) Antibody AG1223-050
    Western blot analysis of acute T-cell leukemia Jurkat (lanes 1 and 4), promyelocytic leukemia HL-60 (lanes 2 and 5) and acute monocytic leukemia THP-1 (lanes 3 and 6) human cell lysates: 1-3. Anti-Human BLT1 (extracellular) antibody (#AG1223), (1:200).
    4-6. Anti-Human BLT1 (extracellular) antibody, preincubated with the control peptide antigen.
  • FC - Human BLT1 (extracellular) Antibody AG1223-050
    Indirect flow cytometry analysis in live intact human T cell leukemia (Jurkat) cell line: Black Control cells + goat-anti-rabbit-Cy5.
    Black Cells + Anti-Human BLT1 (extracellular) antibody (#AG1223), (1:25) + goat-anti-rabbit-Cy5.
  • FC - Human BLT1 (extracellular) Antibody AG1223-050
    Indirect flow cytometry analysis in live intact human acute monocytic leukemia (THP-1) cell line: Black Control cells + goat-anti-rabbit-Cy5.
    Black Cells + Anti-Human BLT1 (extracellular) antibody (#AG1223), (1:25) + goat-anti-rabbit-Cy5.
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q15722
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 37557 Da
Homology Monkey - identical.
Additional Information
Gene ID 1241
Other Names Leukotriene B4 receptor 1, LTB4-R 1, LTB4-R1, Chemoattractant receptor-like 1, G-protein coupled receptor 16, P2Y purinoceptor 7, P2Y7, LTB4R, BLT, BLT1, BLTR, CMKRL1, GPR16, P2RY7
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide CFPRYPSEGHRAFH, corresponding to amino acid residues 168-181 of human BLT1 (Accession Q15722). 2nd extracellular loop.Not recommended for use with rat and mouse samples.
Dilution WB~~1:200-1:2000
FC~~1:25
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution Add 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Background

Leukotrienes (LTs) are lipid mediators prominently exerting proinflammatory responses. They are divided in two classes: cysteinyl LTs (cysLTs) which have a thioester linkage and LTB41.  LTB4, a chemoattractant plays a prominent role in the recruitment and activation of leukocytes2. LTB4 acts through two receptors which belong to the G-protein coupled receptor superfamily: BLT1 and BLT2. BLT1 displays high affinity for LTB4 and is highly expressed in leukocytes including granulocytes, macrophages, mast cells, dendritic cells and effector T cells3 and is expressed to much lower levels in spleen, thymus, heart, skeletal muscle brain and liver2-4. BLT2 on the other hand is a low affinity receptor which does not display selectivity towards LTB45. Its expression is much more ubiquitous than that of BLT1. It is expressed in most human tissues, with the highest expression being in spleen, liver, ovary, and leukocytes2,5-8. The two receptors could couple and activate different G-proteins, depending on the cell type and the cellular events activating the receptors2. However, activation of BLT1 ususally correlates with increasing intracellular concentrations of Ca2+. The LTB4/BLT1 system is involved in many allergic reactions as well as asthma induced as an allergic response, where it plays a significant role in recruiting neutrophils and effector T cells into lungs, as part of an inflammatory response induced by allergens1. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of human BLT1. Anti-Human BLT1 (extracellular) antibody (#AG1223) can be used for Western blot analysis and indirect flow cytometry applications. It has been designed to recognize BLT1 from human samples only.

References

References 1. Riccioni, G. et al. (2008) J. Leukoc. Biol. 84, 1374. 2. Tager, A.M. and Luster A.D. (2003) Prostaglandins Leukot. Essent. Fatty Acids 69, 123. 3. Yokomizo, T. et al. (1997) Nature 387, 620. 4. Owman, C. et al. (1996) Genomics 37, 187. 5. Yokomizo, T. et al. (2000) J. Exp. Med. 192, 421. 6. Tryselius, Y. et al. (2000) Biochem. Biophys. Res. Commun. 274, 377. 7. Kamohara, M. et al. (2000) J. Biol. Chem. 275, 27000. 8. Wang, S. et al. (2000) J. Biol. Chem. 275, 39482.

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$ 475.00
$ 575.00
Cat# AG1223-050
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