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Human H4 Histamine Receptor (extracellular) Antibody

Affinity purified rabbit polyclonal antibody

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  • FC - Human H4 Histamine Receptor (extracellular) Antibody AG1224-050
    Flow cytometric analysis of Jurkat cells using Park7 (DJ-1) Antibody(green, Cat#AG1224) compared to an isotype control of rabbit IgG(blue). AG1224 was diluted at 1:35 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • WB - Human H4 Histamine Receptor (extracellular) Antibody AG1224-050
    Western blot analysis of lysates from HL-60, A431, U-937 cell line (from left to right), using HRH4 Antibody(Cat. #AG1224). AG1224 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • WB - Human H4 Histamine Receptor (extracellular) Antibody AG1224-050
    Western blot analysis of human chronic myelogenous leukemia (K562) (lanes 1 and 3) and human promyelocytic leukemia (HL-60) (lanes 2 and 4) cell lysates: 1, 2. Anti-Human H4 Histamine Receptor (extracellular) antibody (#AG1224), (1:400). 3, 4. Anti-Human H4 Histamine Receptor (extracellular) antibody, preincubated with the control peptide antigen.
  • IF - Human H4 Histamine Receptor (extracellular) Antibody AG1224-050
    Indirect flow cytometry analysis of H4 Histamine Receptor expression in live intact Jurkat (acute T-cell leukemia) cell lines
    Black: Unstained cells + goat-anti-rabbit-PE.
    Green: Cells+ Anti-Human H4 Histamine Receptor (extracellular) antibody (#AG1224), (1:20) + goat-anti-rabbit-PE.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9H3N8
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 44496 Da
Homology Won't recognize rat or mouse H4 Histamine Receptor.
Additional Information
Gene ID 59340
Other Names Histamine H4 receptor, H4R, HH4R, AXOR35, G-protein coupled receptor 105, GPRv53, Pfi-013, SP9144, HRH4, GPCR105
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide HTLFEWDFGKEIC, corresponding to amino acids 75-87 of human H4 Histamine Receptor (Accession Q9H3N8). 1st extracellular loop.
Dilution FC~~1:35
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.7 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1 The biological effects of histamine are mediated through four receptors (H1- H4 Histamine receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. H4 Histamine Receptor couples to Gi/G0 proteins and receptor activation leads to inhibition of adenylate cyclase, mobilisation of calcium from intracellular stores and activation of the mitogen-activated protein kinase (MAPK) cascade. 1, 2 H4 Histamine Receptor is largely expressed in haemopoietic cells including mast cells, eosinophils, dendritic cells and T lymphocytes. H4 Histamine Receptors modulate eosinophil migration and the selective recruitment of mast cells leading to amplification of histamine-mediated immune responses. In addition, H4 Histamine Receptors are involved in dendritic cell activation and the regulation of T lymphocyte cytokine production.2, 3 These studies indicate that H4 Histamine Receptor is an attractive therapeutic target for the treatment of inflammatory disorders, such as allergy, asthma, chronic pruritus and autoimmune diseases.2, 3 Abgent is pleased to offer a highly specific antibody directed against an epitope located at the extracellular 1st loop of the human H4 Histamine receptor. Anti-Human H4 Histamine Receptor (extracellular) antibody (#AG1224) can be used in western blot and indirect flow cytometry applications, and recognizes H4 Histamine receptor from human samples.


1. Hough, L.B (2001) Mol. Pharmacol. 59, 415.
2. Thurmund, R.L. et al. (2008) Nat. Rev. Drug. Discov. 7, 41.
3. Zampeli, E. and Tiligada, E. (2009) Br. J. Pharmacol. 157. 24.

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$ 475.00
$ 575.00
Cat# AG1224-050
(40 western blots)
Availability: 2-3 weeks
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