|Application ||FC, WB|
|Calculated MW||44496 Da|
|Homology||Won't recognize rat or mouse H4 Histamine Receptor.|
|Other Names||Histamine H4 receptor, H4R, HH4R, AXOR35, G-protein coupled receptor 105, GPRv53, Pfi-013, SP9144, HRH4, GPCR105|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide HTLFEWDFGKEIC, corresponding to amino acids 75-87 of human H4 Histamine Receptor (Accession Q9H3N8). 1st extracellular loop.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.7 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1 The biological effects of histamine are mediated through four receptors (H1- H4 Histamine receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. H4 Histamine Receptor couples to Gi/G0 proteins and receptor activation leads to inhibition of adenylate cyclase, mobilisation of calcium from intracellular stores and activation of the mitogen-activated protein kinase (MAPK) cascade. 1, 2 H4 Histamine Receptor is largely expressed in haemopoietic cells including mast cells, eosinophils, dendritic cells and T lymphocytes. H4 Histamine Receptors modulate eosinophil migration and the selective recruitment of mast cells leading to amplification of histamine-mediated immune responses. In addition, H4 Histamine Receptors are involved in dendritic cell activation and the regulation of T lymphocyte cytokine production.2, 3 These studies indicate that H4 Histamine Receptor is an attractive therapeutic target for the treatment of inflammatory disorders, such as allergy, asthma, chronic pruritus and autoimmune diseases.2, 3 Abgent is pleased to offer a highly specific antibody directed against an epitope located at the extracellular 1st loop of the human H4 Histamine receptor. Anti-Human H4 Histamine Receptor (extracellular) antibody (#AG1224) can be used in western blot and indirect flow cytometry applications, and recognizes H4 Histamine receptor from human samples.
1. Hough, L.B (2001) Mol. Pharmacol. 59, 415.
2. Thurmund, R.L. et al. (2008) Nat. Rev. Drug. Discov. 7, 41.
3. Zampeli, E. and Tiligada, E. (2009) Br. J. Pharmacol. 157. 24.
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