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Affinity purified rabbit polyclonal antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, FC |
|---|---|
| Primary Accession | P25102 |
| Reactivity | Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 40253 Da |
| Homology | Mouse - identical. |
| Gene ID | 25461 |
|---|---|
| Other Names | Histamine H2 receptor, H2R, HH2R, Gastric receptor I, Hrh2 |
| Related products for control experiments | Control peptide antigen (supplied with the antibody free of charge). |
| Target/Specificity | Peptide RNGTRGGNDTFKC, corresponding to amino acids 161-173 of rat H2 Histamine Receptor (Accession P25102). 2nd extracellular loop.Will not recognize human H2 Histamine Receptor. |
| Dilution | WB~~1:200-1:2000 IHC~~1:100 IF~~1:20 |
| Peptide Confirmation | Confirmed by amino acid analysis. |
| Format | Affinity purified antibody, lyophilized powder |
| Reconstitution | 50 µl or 0.2 ml deionized water, depending on the sample size. |
| Antibody Concentration After Reconstitution | 0.8 mg/ml. |
| Buffer After Reconstitution | Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3. |
| Storage Before Reconstitution | Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. |
| Storage After Reconstitution | The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min). |
| Control Antigen Storage Before Reconstitution | Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. |
| Control Antigen Reconstitution | 100 µl water |
| Control Antigen Storage After Reconstitution | -20ºC. |
| Preadsorption Control | 1 µg peptide per 1 µg antibody. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1,2 The biological effects of histamine are mediated through four receptors (H1- H4 Histamine receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. H2 receptors couple to adenylate cyclase via Gs, leading to the formation of cAMP and the activation of protein kinase A (PKA).1,2 H2 receptors are expressed in several tissues including brain, gastrointestinal tract and heart. The most studied physiological role of the H2 receptors is their involvement in the regulation of gastric acid secretion.3 Specific H2 receptor antagonists are currently used for the treatment of acid-related gastrointestinal conditions such as peptic ulcer, gastroesophageal reflux disease and dyspepsia. Involvement of H2 receptors in chronic heart failure and immune regulation has also been suggested. Abgent is pleased to offer a highly specific antibody directed against an epitope located at the 2nd extracellular loop of the rat H2 Histamine receptor. Anti-Rat H2 Histamine Receptor (extracellular) antibody (#AG1226) can be used in Western blot analysis, indirect flow cytometry and immunohistochemical applications, and recognizes H2 Histamine receptor from rat and mouse samples.
References
1. Hill, S.J., (1997) Pharmacol. Rev., 49, 253.
2. Del Valle, J. and Gantz, I. (1997) Am. J. Physiol. Gastrointest. Liver Physiol., 273, 987.
3. Kobayashi, T. et al. (2000) J. Clin Invest., 105, 1741.
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