|Application ||WB, IHC|
|Calculated MW||55693 Da|
|Homology||Mouse - identical; human, dog - 13/14 amino acid residues identical.|
|Other Names||Histamine H1 receptor, H1R, HH1R, Hrh1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)HSRQYVSGLHLNRE, corresponding to amino acids 396-409 of rat H1 Histamine Receptor (Accession P31390). 3rd intracellular loop.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1 The biological effects of histamine are mediated through four receptors (H1- H4 Histamine receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. H1 receptors couple to Gq/G11 proteins leading to phospholipase C activation, inositol phosphate production and calcium mobilization.1 H1 receptors are expressed in many cell types including endothelial and smooth muscle cells where they mediate vasodilatation and increased vascular permeability effects. H1 receptors are widely distributed through the brain where the mediate sleep-wake cycles and regulate cognitive functions. H1 receptors are expressed in a several cell populations involved in the immune response, including T-cells and B-cells, monocytes and eosinophiles. 2,3 H1 anti-histamine compounds have been available for almost thirty years and are among the most commonly used medication for conditions such as allergic inflammation. In addition, H1 anti-histamines are also prescribed for the treatment of insomnia and analgesia.2, 3 Abgent is pleased to offer a highly specific antibody directed against an epitope located at the intracellular 3rd loop of the rat H1 Histamine receptor. Anti-H1 Histamine Receptor antibody (#AG1227) can be used in Western blot analysis and immunohistochemical applications, and recognizes H1 Histamine receptor from rat, mouse and human samples.
1. Hill, S.J., (1997) Pharmacol. Rev., 49, 253
2. Thurmund, R.L. et al. (2008) Nat. Rev. Drug. Discov. 7, 41.
3. Haas, H.L. et al. (2008) Physiol. Rev., 88, 1183.
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