|Reactivity||Human, Mouse, Rat|
|Calculated MW||57407 Da|
|Homology||Mouse, human - identical.|
|Other Names||Vesicular inhibitory amino acid transporter, GABA and glycine transporter, Solute carrier family 32 member 1, Vesicular GABA transporter, rGVAT, Slc32a1, Vgat, Viaat|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)GEFGGHDKPKITAWE, corresponding to amino acid residues 106-120 of rat vesicular GABA transporter (Accession O35458). Cytoplasmic, N-terminus|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||1 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody|
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Provided below are standard protocols that you may find useful for product applications.
The superfamily of amino acid transporters includes SLC32, SLC36 and SLC38 families. SLC32 has only one family member, the vesicular inhibitory amino acid transporter (VIAAT) or vesicular GABA transporter (VGAT)1. VGAT is responsible for the uptake of GABA and Glycine in exchange of protons, and their release from nerve terminals1. The transporter is organized into ten membrane spanning domains, a cytosolic N-terminus and a luminal C-terminal tail2. In many regions in the brain, where it is expressed, it is found constitutively phosphorylated, although the biological significance is currently unknown3. VGAT is expressed on synaptic vesicles of GABAergic and glycynergic neurons4-6. The transporter is also detected in the pituitary gland and in the pancreas7,8. Abgent is pleased to offer a highly specific antibody directed against an epitope of rat vesicular GABA transporter. Anti-Vesicular GABA Transporter antibody (#AG1231) can be used in western blot analysis. It has been designed to recognize VGAT from human, mouse and rat samples.
References 1. Gasnier, B. (2004) Pflugers Arch. 447, 756. 2. McIntire, S. et al. (1997) Nature 389, 870. 3. Bedet, C. et al. (2000) J. Neurochem. 75, 1654. 4. Chaudry, F. et al. (1998) J. Neurosci. 18, 9733. 5. Dumoulin, A. et al. (1999) J. Cell Sci. 112, 811. 6. Takamori, S. et al. (2000) J. Neurosci. 20, 4904. 7. Mayerhofer, A. et al. (2001) FASEB J. 15, 1089. 8. Chessler, S. et al. (2002) Diabetes 51, 1763.
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