|Calculated MW||69947 Da|
|Other Names||Sodium- and chloride-dependent GABA transporter 3, GAT-3, Solute carrier family 6 member 11, Slc6a11, Gabt3, Gat-3, Gat-b|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)REARDKAVHERGH, corresponding to amino acid residues 35- 47 of rat GABA Transporter 3 (Accession P31647). Intracellular, N-terminus.Not recommended for use with human samples.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
γ-aminobutiric acid (GABA) is the major inhibitory neurotransmitter in the mammalian central nervous system. Transmission via GABA can be halted by its reuptake through GABA transporters (GATs). These membrane proteins belong to the super family of slc6 genes which encode transporters responsible for the uptake of dopamine, serotonin, norepinephrine, glycine and GABA in a Na+-dependent manner1,2. To date, four GATs have been identified: GAT-1, GAT-2, GAT-3 and BGT-1. Like all members of the slc6 family, they have twelve transmembrane domains and short intracellular N- and C-termini1. GATs couple the transport of one GABA molecule to two Na+ ions and one Cl- ion3. GAT-3 is highly expressed in the brain and is linked to epilepsy4. Abgent is pleased to offer a highly specific antibody directed against an epitope of rat GABA Transporter 3. Anti-GABA Transporter 3 (GAT-3) antibody (#AG1232) can be used in western blot analysis, and has been designed to recognize GAT-3 from rat and mouse samples.
References 1. Gonzalez-Burgos, G. (2010) Adv. Pharmacol. 58, 175. 2. Gether, U. et al. (2006) Trends Pharmacol. Sci. 27, 375. 3. Meinild, A.K. et al. (2009) J. Biol. Chem. 284, 16226. 4. Kristensen, A.S. et al. (2011) Pharmacol. Rev. 63, 585.
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