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GABA Transporter 2 (GAT-2) Antibody

Affinity purified polyclonal antibody

  • WB - GABA Transporter 2 (GAT-2) Antibody AG1233-050
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: 1, 2. Anti-GABA Transporter 2 (GAT-2) antibody (#AG1233), (1:200).
    3, 4. Anti-GABA Transporter 2 (GAT-2) antibody, preincubated with the control peptide antigen.
  • IHC - GABA Transporter 2 (GAT-2) Antibody AG1233-050
    Expression of GABA Transporter 2 (GAT-2) in rat lung Immunohistochemical staining of rat lung paraffin-embedded sections using Anti-GABA Transporter 2 (GAT-2) antibody (#AG1233), (1:100). GAT-2 (brown) is expressed in the respiratory epithelium of the bronchioli. Hematoxilin is used as the counterstain.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P31646
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 68263 Da
Homology Mouse - 14/16 amino acid residues identical; human - 11/16 amino acid residues identical.
Additional Information
Gene ID 171163
Other Names Sodium- and chloride-dependent GABA transporter 2, GAT-2, Solute carrier family 6 member 13, Slc6a13, Gabt2, Gat-2
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)KVEEDGTLEREQWTNK, corresponding to amino acids residues 23-38 of rat GABA Transporter ֲ 2 (Accession P31646). Intracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
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γ-aminobutiric acid (GABA) is the major inhibitory neurotransmitter in the mammalian central nervous system. Transmission via GABA can be halted by its reuptake through GABA transporters (GATs). These membrane proteins belong to the super family of slc6 genes which encode transporters responsible for the uptake of dopamine, serotonin, norepinephrine, glycine and GABA in a Na+-dependent manner1,2. To date, four GATs have been identified: GAT-1, GAT-2, GAT-3 and BGT-1. Like all members of the slc6 family, they have twelve transmembrane domains and short intracellular N- and C-termini1. GATs couple the transport of one GABA molecule to two Na+ ions and one Cl- ion3. GAT-2 is expressed in the brain and in the periphery and is also abundant in the kidney and liver4. Due to its relatively broad expression pattern, suggested roles for GAT-2 include maturation of the nervous system, and regulation of GABA levels among others4. Abgent is pleased to offer a highly specific antibody directed against an epitope of rat GABA Transporter 2. Anti-GABA Transporter 2 (GAT-2) antibody (#AG1233) can be used in western blot analysis and immunohistochemistry applications and has been designed to recognize GAT-2 from rat, mouse and human samples.


References 1. Gonzalez-Burgos, G. (2010) Adv. Pharmacol. 58, 175. 2. Gether, U. et al. (2006) Trends Pharmacol. Sci. 27, 375. 3. Meinild, A.K. et al. (2009) J. Biol. Chem. 284, 16226. 4. Christiansen, B. et al. (2007) J. Biol. Chem. 282, 19331.

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$ 475.00
$ 575.00
Cat# AG1233-050
(40 western blots)
Availability: 2-3 weeks
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