|Calculated MW||68263 Da|
|Homology||Mouse - 14/16 amino acid residues identical; human - 11/16 amino acid residues identical.|
|Other Names||Sodium- and chloride-dependent GABA transporter 2, GAT-2, Solute carrier family 6 member 13, Slc6a13, Gabt2, Gat-2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)KVEEDGTLEREQWTNK, corresponding to amino acids residues 23-38 of rat GABA Transporter ֲ 2 (Accession P31646). Intracellular, N-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
γ-aminobutiric acid (GABA) is the major inhibitory neurotransmitter in the mammalian central nervous system. Transmission via GABA can be halted by its reuptake through GABA transporters (GATs). These membrane proteins belong to the super family of slc6 genes which encode transporters responsible for the uptake of dopamine, serotonin, norepinephrine, glycine and GABA in a Na+-dependent manner1,2. To date, four GATs have been identified: GAT-1, GAT-2, GAT-3 and BGT-1. Like all members of the slc6 family, they have twelve transmembrane domains and short intracellular N- and C-termini1. GATs couple the transport of one GABA molecule to two Na+ ions and one Cl- ion3. GAT-2 is expressed in the brain and in the periphery and is also abundant in the kidney and liver4. Due to its relatively broad expression pattern, suggested roles for GAT-2 include maturation of the nervous system, and regulation of GABA levels among others4. Abgent is pleased to offer a highly specific antibody directed against an epitope of rat GABA Transporter 2. Anti-GABA Transporter 2 (GAT-2) antibody (#AG1233) can be used in western blot analysis and immunohistochemistry applications and has been designed to recognize GAT-2 from rat, mouse and human samples.
References 1. Gonzalez-Burgos, G. (2010) Adv. Pharmacol. 58, 175. 2. Gether, U. et al. (2006) Trends Pharmacol. Sci. 27, 375. 3. Meinild, A.K. et al. (2009) J. Biol. Chem. 284, 16226. 4. Christiansen, B. et al. (2007) J. Biol. Chem. 282, 19331.
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