|Reactivity||Human, Mouse, Rat|
|Calculated MW||67001 Da|
|Homology||Mouse, human - identical.|
|Other Names||Sodium- and chloride-dependent GABA transporter 1, GAT-1, Solute carrier family 6 member 1, Slc6a1, Gabt1, Gat-1, Gat1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)ERNMHQMTDGLDK, corresponding to amino acids residues 194-206 of rat GABA Transporterֲ 1 (Accession P23978). 2ndֲ extracellular loop.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
γ-aminobutiric acid (GABA) is the major inhibitory neurotransmitter in the mammalian central nervous system. Transmission via GABA can be halted by its reuptake through GABA transporters (GATs). These membrane proteins belong to the super family of slc6 genes which encode transporters responsible for the uptake of dopamine, serotonin, norepinephrine, glycine and GABA in a Na+-dependent manner1,2. To date, four GATs have been identified: GAT-1, GAT-2, GAT-3 and BGT-1. Like all members of the slc6 family, they have twelve transmembrane domains and short intracellular N- and C-termini1. GATs couple the transport of one GABA molecule to 2Na+ ions and one Cl- ion3. GAT-1 activity can be regulated at the level of its plasma membrane expression which is in part regulated by its trafficking via phosphorylation of residues in intracellular loops1. GAT-1 expression is restricted to the central nervous system, specifically to axon terminals, in the neocortex and in some astrocytic processes4. GAT-1 may be implicated in various psychological and neurological disorders such as schizophrenia, epilepsy and cerebral ischemia1,5-7. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat GABA Transporter 1. Anti-GABA Transporter 1 (GAT-1) (extracellular) antibody (#AG1234) can be used in western blot analysis, and has been designed to recognize GAT-1 from rat, mouse and human samples.
References 1. Gonzalez-Burgos, G. (2010) Adv. Pharmacol. 58, 175. 2. Gether, U. et al. (2006) Trends Pharmacol. Sci. 27, 375. 3. Meinild, A.K. et al. (2009) J. Biol. Chem. 284, 16226. 4. Conti, F. et al. (2011) Front. Cell. Neurosci. 5, 1. 5. Lewis, D.A. et al. (2005) Nat. Rev. Neurosci. 6, 312. 6. Fueta, Y. et al. (2003) Neuroscience 118, 371. 7. Frahm, C. et al. (2004) NeuroReport 15, 9.
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