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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   GABA Transporter 1 (GAT-1) (extracellular) Antibody   

GABA Transporter 1 (GAT-1) (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - GABA Transporter 1 (GAT-1) (extracellular) Antibody AG1234-025
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    1, 2.  Anti-GABA Transporter 1 (GAT-1) (extracellular) antibody (#AG1234), (1:200).
    3, 4.  Anti-GABA Transporter 1 (GAT-1) (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - GABA Transporter 1 (GAT-1) (extracellular) Antibody AG1234-025
    Expression of GABA Transporter 1 (GAT-1) in rat cerebellum Immunohistochemical staining of rat cerebellum using Anti-GABA Transporter 1 (GAT-1) (extracellular) antibody (#AG1234), (1:200). A. GAT-1 staining is shown in green. B. Neurons expressing gamma amino butyric acid (GABA) were labeled with mouse Anti -parvalbumin (red). C. Merge of the two images demonstrates partial colocalization (arrows). GAT-1 appears both in the soma of Purkinje cells (vertical arrows) and in the pinceau (horizontal arrows).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P23978
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 67001 Da
Homology Mouse, human - identical.
Additional Information
Gene ID 79212
Other Names Sodium- and chloride-dependent GABA transporter 1, GAT-1, Solute carrier family 6 member 1, Slc6a1, Gabt1, Gat-1, Gat1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)ERNMHQMTDGLDK, corresponding to amino acids residues 194-206 of rat GABA Transporterֲ  1 (Accession P23978). 2ndֲ  extracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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γ-aminobutiric acid (GABA) is the major inhibitory neurotransmitter in the mammalian central nervous system. Transmission via GABA can be halted by its reuptake through GABA transporters (GATs). These membrane proteins belong to the super family of slc6 genes which encode transporters responsible for the uptake of dopamine, serotonin, norepinephrine, glycine and GABA in a Na+-dependent manner1,2. To date, four GATs have been identified: GAT-1, GAT-2, GAT-3 and BGT-1. Like all members of the slc6 family, they have twelve transmembrane domains and short intracellular N- and C-termini1. GATs couple the transport of one GABA molecule to 2Na+ ions and one Cl- ion3. GAT-1 activity can be regulated at the level of its plasma membrane expression which is in part regulated by its trafficking via phosphorylation of residues in intracellular loops1. GAT-1 expression is restricted to the central nervous system, specifically to axon terminals, in the neocortex and in some astrocytic processes4. GAT-1 may be implicated in various psychological and neurological disorders such as schizophrenia, epilepsy and cerebral ischemia1,5-7. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat GABA Transporter 1. Anti-GABA Transporter 1 (GAT-1) (extracellular) antibody (#AG1234) can be used in western blot analysis, and has been designed to recognize GAT-1 from rat, mouse and human samples.


References 1. Gonzalez-Burgos, G. (2010) Adv. Pharmacol. 58, 175. 2. Gether, U. et al. (2006) Trends Pharmacol. Sci. 27, 375. 3. Meinild, A.K. et al. (2009) J. Biol. Chem. 284, 16226. 4. Conti, F. et al. (2011) Front. Cell. Neurosci. 5, 1. 5. Lewis, D.A. et al. (2005) Nat. Rev. Neurosci. 6, 312. 6. Fueta, Y. et al. (2003) Neuroscience 118, 371. 7. Frahm, C. et al. (2004) NeuroReport 15, 9.

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$ 375.00
$ 475.00
$ 575.00
Cat# AG1234-025
(40 western blots)
Availability: 2-3 weeks
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