|Application ||WB, LCI|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||40963 Da|
|Homology||Mouse - identical; human - 10/11 amino acid residues identical.|
|Other Names||Growth hormone secretagogue receptor type 1, GHS-R, GH-releasing peptide receptor, GHRP, Ghrelin receptor, Ghsr|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide CRATEFAVRSG, corresponding to amino acid residues ֲ 197-207 of rat Ghrelin Receptor (Accession O08725). 2nd extracellular loop.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||1 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Ghrelin is a 28 amino acid peptide, acylated on a serine residue which is essential for its biological activity1,2. In rodents, an additional form of the peptide could be purified. The peptide is released from the stomach and exerts its effect to ghrelin receptor (GHS-R). GHS-R is a member of the G-protein coupled receptor (GPCR) superfamily. Like all members, the receptor has seven transmembrane spanning domains and upon activation it stimulates phospholipase C, thereby increasing IP3 and intracellular Ca2+ levels1,3. GHS-R is expressed in the hypothalamus, pituitary and hippocampus1,3. Some of the actions of ghrelin include the stimulation of growth hormone release, food intake, fat accumulation, regulation of memory and learning, activation of reward-related pathways and neuroprotection to name a few1,3. GHS-R 1b is a truncated form of the full length receptor which is also expressed. Although its exact functions are unknown, it is believed that it may be involved in downregulating signaling from the full length receptor4. In addition, it is overexpressed in lung cancer where it dimerizes with neurotensin receptor4,5. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat Ghrelin receptor. Anti-Ghrelin Receptor (extracellular) antibody (#AG1235) can be used in western blot and immunocytochemistry applications. It has been designed to recognize ghrelin receptor from human mouse and rat samples.
References 1. Sato, T. et al. (2012) J. Biochem. 151, 119. 2. Kojima, M. et al. (1999) Nature 402, 656. 3. Albarran-Zeckler, R.G. et al. (2011) Peptides 32, 2229. 4. Seim, I. et al. (2011) Mol. Cell. Endo. 340, 3. 5. Takahashi, K. et al. (2006) Cancer Res. 66, 9408.
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