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GnRH Receptor (extracellular) Antibody

Affinity purified rabbit polyclonal antibody

  • WB - GnRH Receptor (extracellular) Antibody AG1236-050
    Western blot analysis of rat ovary (lanes 1 and 3) and rat pituitary (lanes 2 and 4) lysates: 1, 2. Anti-GnRH Receptor (extracellular) antibody (#AG1236), (1:200). 3, 4. Anti-GnRH Receptor (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - GnRH Receptor (extracellular) Antibody AG1236-050
    Expression of GnRH in rat ovary Immunohistochemical staining of rat ovary paraffin-embedded sections using Anti-GnRH Receptor (extracellular) antibody (#AG1236). GnRHR labeling appears in the stroma surrounding the follicles. The stroma is a richly cellular connective tissue with scattered smooth muscle fibers. The Theca layer is stained as well. Hematoxilin is used as the counterstain.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P30969
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 37748 Da
Homology Mouse - identical.
Additional Information
Gene ID 81668
Other Names Gonadotropin-releasing hormone receptor, GnRH receptor, GnRH-R, Gnrhr
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)NNASLEQDQNH, corresponding to amino acid residues 3-13 of rat GnRH Receptor (Accession P30969).  Extracellular, N-terminus.Not recommended for use with human samples.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis and mass-spectrography.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.85 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
Citations (0)

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Gonadotropin-releasing hormone (GnRH) or Luteinizing hormone releasing hormone (LHRH) is a decapeptide synthesized and released from neurons in the hypothalamus and stimulates the synthesis and release of gonadotropin hormone (or luteinizing hormone) and follicle stimulating hormone from the pituitary gland1. GnRH is expressed as various variants, and most vertebrates express more than one form of GnRH1-3. GnRH and its variants bind and activate GnRH receptor (GnRHR), a member of the G-protein coupled receptor (GPCR) superfamily. Three forms of the receptor have been identified in vertebrates (types I, II and III). Like all members, GnRHR has seven transmembrane domains and an extracellular N-terminus and an intracellular C-terminal tail. The receptor also has a number of glycosylation sites in its extracellular loops. In addition, the C-terminal tail is unusually short and lacks consensus sites known to undergo post-translational modifications like phosphorylation. In consequence, this affects the receptor’s desensitization and internalization4. Expression of GnRHR is detected in the gonadotrope and various tissues like breast, gonads, prostate and uterus4,5. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat GnRH receptor. Anti-GnRH Receptor (extracellular) antibody (#AG1236) can be used in western blot and immunohistochemistry applications. It has been designed to recognize GnRHR from mouse and rat samples.


1. Naor, Z. (2009) Front. Neuroendocrinol. 30, 10.
2. Millar, R.P. et al. (2004) Endocr. Rev. 25, 235.
3. Tello, J.A. et al. (2005) Endocrinology 146, 4061.
4. Bliss, S.P. et al. (2010) Front. Neuroencrinol. 31, 322.
5. Cheung, L.W. and Wong, A.S. (2008) FEBS J. 275, 5479.

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$ 475.00
$ 575.00
Cat# AG1236-050
(40 western blots)
Availability: 2-3 weeks
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