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Affinity purified polyclonal antibody
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- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | Q9Z0W0 |
| Reactivity | Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 63102 Da |
| Homology | Mouse, human - 13/15 amino acid residues identical. |
| Gene ID | 60432 |
|---|---|
| Other Names | Glucagon-like peptide 2 receptor, GLP-2 receptor, GLP-2-R, GLP-2R, Glp2r |
| Related products for control experiments | Control peptide antigen (supplied with the antibody free of charge). |
| Target/Specificity | Peptide (C)KRPDDESGWMSYLSE, corresponding to amino acid residues 241-255 of rat GLP2R (Accession Q9Z0W0). 1st extracellular loop. |
| Dilution | WB~~1:200-1:2000 IHC~~1:200 |
| Peptide Confirmation | Confirmed by amino acid analysis. |
| Format | Affinity purified antibody, lyophilized powder |
| Reconstitution | Add 50 µl or 0.2 ml deionized water, depending on the sample size. |
| Antibody Concentration After Reconstitution | 0.8 mg/ml. |
| Buffer After Reconstitution | Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
| Storage Before Reconstitution | Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. |
| Storage After Reconstitution | The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min). |
| Control Antigen Storage Before Reconstitution | Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. |
| Control Antigen Reconstitution | 100 µl water. |
| Control Antigen Storage After Reconstitution | -20ºC. |
| Preadsorption Control | 1 µg peptide per 1 µg antibody. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Glucagon like peptide-2 (GLP-2) is a 33 amino acid peptide secreted following food consumption. It is cleaved by dipeptidyl peptidase IV thereby yielding a very short half life. GLP-2 imposes most of its effect on intestinal epithelial cells where it is also be used as a therapeutic drug for gastrointestinal injuries1-3. The many actions of GLP-2 include cellular proliferation of intestinal epithelial cells, the increase of amino acid and lipid absorption and increase of digestive enzyme activity of the epithelial brush border cells1. The GLP-2 cellular and physiological effects are primarily exerted through the glucagon-like peptide-2 receptor (GLP-2R), a member of the G-protein coupled receptor superfamily. The receptor can couple either Gs or Gi G-proteins1. However, when heterologously expressed, the receptor can stimulate the synthesis of cAMP4. Expression of GLP-2R is strongly detected in the central nervous system and the gastrointestinal tract, although its expression in this area remains controversial as some species express the receptor in different regions than others. It is also slightly detected in the lungs, cervix and vagal afferents1. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat Glucagon-like peptide 2 receptor. Anti-Glucagon-like Peptide 2 Receptor (extracellular) antibody (#AG1237) can be used for western blot analysis and immunohistochemical applications. It has been designed to recognize Glucagon-like peptide 2 receptor from human, rat and mouse samples.
References
References 1. Dube, P.E. and Brubaker, P.L. (2007) Am. J. Physiol. 283, E460. 2. Dube, P.E. and Brubaker, P.L. (2007) Am. J. Physiol. 283, E460. 3. Wallis, K. et al. (2006) Aliment. Pharmacol. Ther. 25, 365. 4. Mayo, K.E. et al. (2003) Pharmacol. Rev. 55, 167.
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