|Reactivity||Human, Mouse, Rat|
|Calculated MW||52877 Da|
|Homology||Rat, mouse, human - 13/14 amino acid residues identical.|
|Other Names||Glucagon-like peptide 1 receptor, GLP-1 receptor, GLP-1-R, GLP-1R, Glp1r, Glpr|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)KYLYEDEG(S)WTRNS, corresponding to amino acid residues 288-301 of rat GLP1R (Accession P32301). 2nd extracellular loop.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||Add 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Glucagon-like peptide 1 (GLP-1) is a hormone secreted by the intestine following a meal1. This hormone is known to enhance the secretion of insulin, important to regulate glucose blood levels2,3. In pancreatic b-cells, other than stimulating insulin secretion, GLP-1 action is also important for b-cell mass expansion2. GLP-1 exerts its biological functions through Glucagon-like peptide 1 receptor (GLP-1 receptor), a member of the G-protein coupled receptor superfamily. Like all members this receptor spans the membrane seven times. GLP-1 receptor couples with Gs, thereby stimulates adenylate cyclase which consequently leads to an increase in cAMP2. The receptor is expressed in various tissues, namely the pancreas, adipose tissue, muscle, heart, gastrointestinal tract and liver. GLP-1 receptor is also found in the central nervous system where it provides a neuroprotective effect4. The glucagon-like peptide 1 system may serve as a therapeutic treatment in diabetes mellitus, in order to maintain b-cell mass expansion2. In addition, the receptor may serve as a therapy target in various neurodegenerative diseases like Alzheimer’s and Parkinson’s disease4. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat Glucagon-like peptide 1 receptor. Anti-Glucagon-like Peptide 1 Receptor (extracellular) antibody (#AG1238) can be used for western blot, immunohistochemical and immunocytochemical applications. It has been designed to recognize Glucagon-like peptide 1 receptor from human, rat and mouse samples.
References 1. Donath, M.Y. et al. (2003) J. Mol. Med. 81, 455. 2. Buteau, J. (2008) Diabetes Metab. 34, S73. 3. Holst, J.J. (2007) Physiol. Rev. 87, 1409. 4. Harkavyi, A. and Whitton, P.S. (2010) Br. J. Pharmacol. 159, 495.
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