|Calculated MW||40420 Da|
|Homology||Rat, human - identical.|
|Other Names||Galanin receptor type 3, GAL3-R, GALR-3, Galr3, Galnr3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide SYYGTVRYGALELC, corresponding to amino acid residues 159-172 of mouse Galanin receptor type 3 (Accession O88853). 2nd extracellular loop.|
|Peptide Confirmation||Confirmed by amino acid analysis and mass-spectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.7 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Galanin is a neuropeptide, consisting of 29-30 amino acid residues, involved in the regulation of cognition, mood, feeding, seizure activity and pain. Galanin exerts its action via three known Galanin receptors, the Galanin receptor type 1 (GALR1), Galanin receptor type 2 (GALR2) and Galanin receptor type 3 (GALR3). The Galanin receptors belong to the G-protein-coupled receptor superfamily.1-4 Based on mRNA, the GALR1 is predominantly distributed in the central nervous system, spinal cord and dorsal root ganglia while GALR2 is abundant in both central and peripheral nervous system. GALR3 mRNA is distributed in peripheral tissues such as heart, spleen, and testis. However, its expression in the brain is still controversial.4,5 Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of mouse Galanin Receptor type 3 (GALR3). Anti-Galanin Receptor Type 3 (extracellular) antibody (#AG1239) can be used for Western blot analysis and immunohistochemistry applications. It has been designed to recognize GALR3 from mouse, rat and human samples.
References 1. Branchek, T.A. et al. (2000) Trends Pharmacol Sci, 21, 109. 2. Branchek, T.A. et al. (1998) Ann. N. Y. Acad. Sci. 863, 94. 3. Barreda-Go´mez, G. et al. (2005) Neuropeptides 39, 153. 4. Hawes, J.J. et al. (2004) J. Comp. Neurol. 479, 410.
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