|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||40516 Da|
|Homology||Rat - identical; human - 11/13 amino acid residues identical.|
|Other Names||Galanin receptor type 2, GAL2-R, GALR-2, Galr2, Galnr2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide(C)GSGSQRAKRKVTR, corresponding to amino acid residues 223-235 of mouse Galanin receptor type 2 (Accession O88854). 3rd intracellular loop.|
|Peptide Confirmation||Confirmed by amino acid analysis and mass-spectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.75 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Galanin is a neuropeptide, consisting of 29-30 amino acid residues, involved in the regulation of cognition, mood, feeding, seizure activity and pain. Galanin exerts its action via three known Galanin Receptors, the Galanin Receptor Type 1 (GALR1), Galanin Receptor Type 2 (GALR2) and Galanin Receptor Type 3 (GALR3). The Galanin Receptors belong to the G-Protein Coupled Receptor superfamily.1-4 GALR2 is widely expressed in the brain (with highest levels of mRNA found in the hypothalamus, hippocampus, amygdala and pyriform cortex) and in peripheral tissues such as the small intestine, heart, kidney, and liver. GALR2 high levels of expression were also found in rat dorsal root ganglia.1 GALR2 activation increases inositol phosphate hydrolysis, mediates the release of Ca2+ into the cytoplasm from intracellular stores, and opens Ca2+-dependent chloride channels in a PTX-resistant manner, indicating that GALR2 couples to Gq/11 proteins.5 Abgent is pleased to offer a highly specific antibody directed against an intracellular epitope of mouse Galanin Receptor type 2 (GALR2). Anti-Galanin Receptor Type 2 antibody (#AG1240) can be used for Western blot analysis and immunohis
1. Branchek, T.A. et al. (2000) Trends Pharmacol Sci, 21, 109.
2. Branchek, T.A. et al. (1998) Ann. N. Y. Acad. Sci. 863, 94.
3. Barreda-Go´mez, G. et al. (2005) Neuropeptides 39, 153.
4. Lu, X. et al. (2005) Neuropeptides 39, 147.
5. Counts, S.E. et al. (2003) Mol. Interv. 3, 137.
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