|Application ||WB, IHC, ICC|
|Calculated MW||39114 Da|
|Homology||Rat, human - identical.|
|Other Names||Galanin receptor type 1, GAL1-R, GALR-1, Galr1, Galnr, Galnr1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide RSKPGKPRSTTN(C), corresponding to amino acid residues 60-71 of mouse GALR1 (Accession P56479). 1st intracellular loop.|
|Peptide Confirmation||Confirmed by amino acid analysis and mass-spectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Galanin is a neuropeptide, consisting of 29-30 amino acid residues, involved in the regulation of cognition, mood, feeding, seizure activity and pain. Galanin exerts its action via three known Galanin receptors, the Galanin receptor type 1 (GALR1), Galanin receptor type 2 (GALR2) and Galanin receptor type 3 (GALR3). The Galanin receptors belong to the G-protein-coupled receptor superfamily.1-4 Based on mRNA, the GALR1 is predominantly distributed in the central nervous system, spinal cord and dorsal root ganglia while GALR2 is abundant in both central and peripheral nervous system. GALR3 mRNA is distributed in peripheral tissues such as heart, spleen, and testis. However, its expression in the brain is still controversial.4,5 Abgent is pleased to offer a highly specific antibody directed against the 1st intracellular loop of the mouse Galanin Receptor type 1 (GALR1). Anti-Galanin Receptor Type 1 antibody (#AG1241) can be used for Western blot analysis, immunohistochemical and immunocytochemical applications. It has been designed to recognize GALR1 from mouse, rat and human samples.
1. Branchek, T.A. et al. (2000) Trends Pharmacol Sci, 21, 109.
2. Branchek, T.A. et al. (1998) Ann. N. Y. Acad. Sci. 863, 94.
3. Barreda-Go´mez, G. et al. (2005) Neuropeptides 39, 153.
4. Lu, X. et al. (2005) Neuropeptides 39, 147.
5. Hawes, J.J. et al. (2004) J. Comp. Neurol. 479, 410.
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