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Glycine Receptor alpha3 Antibody

Affinity purified polyclonal antibody

  • WB - Glycine Receptor alpha3 Antibody AG1242-050
    Western blot analysis of rat brain membranes: 1. Anti-Glycine Receptor α3 antibody (#AG1242), (1:200).  
    2. Anti-Glycine Receptor α3 antibody, preincubated with the control peptide antigen.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P24524
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 53672 Da
Homology Mouse, human - identical.
Additional Information
Gene ID 114516
Other Names Glycine receptor subunit alpha-3, Glra3
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)KNKTEAFALEKFYR, corresponding to amino acidֲ residues 355-368 of rat Glycine Receptor ־±3 (accession number P24524). 2nd intracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis and mass-spectrography.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Citations (0)

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Glycine is a major neurotransmitter in the mammalian central nervous system.1   The glycine receptor is a ligand gated Cl- channel, mediating synaptic inhibition in the spinal cord, retina and regions of the brain.2   The glycine receptor is a pentamer consisting of α and β subunits.   To date there are four known α-subunit isoforms (α1- α4) encoded by different genes.2-4   In humans, two alternative splice variants of the glycine receptor α3 have been described: α3L, and α3K where the α3L splice variant bears an additional segment of 15 amino acids within the long cytoplasmic TM3-4 loop. These variants differ in their desensitization kinetics.3


References 1. Becker, C.M. (1995) The Neuroscientist 1, 130. 2. selmann, G. et al. (2002) Eur. J. Neurosci. 16, 69. 3. Nikolic, Z. et al. (1998) J. Biol. Chem. 273, 19708. 4. Breitinger, H.G. et al. (2002) J.Neurochem. 83, 30.

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$ 475.00
$ 575.00
Cat# AG1242-050
(40 western blots)
Availability: 2-3 weeks
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