|Calculated MW||53672 Da|
|Homology||Mouse, human - identical.|
|Other Names||Glycine receptor subunit alpha-3, Glra3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)KNKTEAFALEKFYR, corresponding to amino acidֲ residues 355-368 of rat Glycine Receptor ־±3 (accession number P24524). 2nd intracellular loop.|
|Peptide Confirmation||Confirmed by amino acid analysis and mass-spectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Glycine is a major neurotransmitter in the mammalian central nervous system.1 The glycine receptor is a ligand gated Cl- channel, mediating synaptic inhibition in the spinal cord, retina and regions of the brain.2 The glycine receptor is a pentamer consisting of α and β subunits. To date there are four known α-subunit isoforms (α1- α4) encoded by different genes.2-4 In humans, two alternative splice variants of the glycine receptor α3 have been described: α3L, and α3K where the α3L splice variant bears an additional segment of 15 amino acids within the long cytoplasmic TM3-4 loop. These variants differ in their desensitization kinetics.3
References 1. Becker, C.M. (1995) The Neuroscientist 1, 130. 2. selmann, G. et al. (2002) Eur. J. Neurosci. 16, 69. 3. Nikolic, Z. et al. (1998) J. Biol. Chem. 273, 19708. 4. Breitinger, H.G. et al. (2002) J.Neurochem. 83, 30.
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