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mGluR8 (extracellular) Antibody

Affinity purified rabbit polyclonal antibody

  • WB - mGluR8 (extracellular) Antibody AG1247-050
    Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell line lysate (lanes 3 and 6): 1-3. Anti-mGluR8 (extracellular) antibody (#AG1247), (1:400). 4-6. Anti-mGluR8 (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - mGluR8 (extracellular) Antibody AG1247-050
    Expression of mGluR8 in rat globus pallidus Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-mGluR8 (extracellular) antibody (#AG1247), (1:100). A. Staining reveals expression of mGluR8 (red) in globus pallidus neurons (arrow) and their dendrites. B, The same section was also stained with DAPI (blue) revealing cell nuclei. C. Merge of the two images.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P70579
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 101867 Da
Homology Rat, mouse, human ג€“ 12/13 amino acid residues identical.
Additional Information
Gene ID 60590
Other Names Metabotropic glutamate receptor 8, mGluR8, Grm8, Gprc1h, Mglur8
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)ENFG(S)KLGSHGKR, corresponding to amino acid residues 365- 377 of rat mGluR8 (Accession P70579). Extracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Citations (0)

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Metabotropic glutamate receptors (mGluRs), heptahelical transmembrane receptors coupled to G-proteins, contribute to the regulation of neuronal excitability and synaptic transmission1. Genes coding for eight mGluRs (denoted mGluR1-8) with different splice variants have been identified and classified into three groups based on sequence homology, pharmacology, and signaling pathways. Group I mGluRs (mGluR1 and mGluR5) are primarily located postsynaptically. Group II (mGluR2 and mGluR3) are present both postsynaptically and presynaptically outside the release site. Group III mGluRs (mGluR4, mGluR6, mGluR7, and mGluR8) couple to Gαi/o to inhibit adenylyl cyclase found in the presynaptic active zone2. The mGluR8 receptor is detected in the olfactory bulb, thalamus, pontine gray, cerebral cortex, hippocampus, cerebellum, and retina3. Electrophysiological studies suggest that mGluR8 functions as a presynaptic autoreceptor to regulate glutamate release from the lateral perforant path terminals in the mouse dentate gyrus4. It is suggested that mGluR8, like other group III mGluRs, controls glutamate release by inhibiting voltage-gated Ca2+ channels. Targeted deletion of the mGluR8 gene in mice produces subtle behavioral alterations such as reduced habituation to novelty, hyperactivity, context-dependent but not cue-dependent disruption of the freezing response in the fear conditioning test, and increased anxiety5.


1. Conn, P.J. et al. (1997) Annu. Rev. Pharmacol. Toxicol. 37, 205.
2. Somogyi, J. et al. (2004) Eur. J. Neurosci. 19, 552.
3. Saugstad, J.A. et al. (1997) Mol. Pharmacol. 51, 119.
4. Zhai, J. et al. (2002) Neuropharmacology 43, 223.

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$ 475.00
$ 575.00
Cat# AG1247-050
(40 western blots)
Availability: 2-3 weeks
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