|Reactivity||Human, Mouse, Rat|
|Calculated MW||95089 Da|
|Homology||Mouse, rat - 13/14 amino acid residues identical; human - 12/14 amino acid residues identical.|
|Other Names||Metabotropic glutamate receptor 6, mGluR6, Grm6, Gprc1f, Mglur6|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)GQSDDSTRK(S)TGEE, corresponding to amino acid residues 368-381 of rat mGluR6 (Accession P35349). Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Glutamate is the principal excitatory neurotransmitter in the central nervous system. It operates through two main classes of receptors: ionotropic receptors (iGluRs), which are ligand-gated ion channels, and metabotropic receptors (mGluRs), which couple to G-proteins to modulate cellular responses via ion channels or intracellular second messengers. The metabotropic receptors include eight family members (mGluR1-mGluR8) classified into three groups according to their sequence similarities, signal transduction, and agonist selectivity. They all share a common structure, being composed of a single polypeptide with large N-terminal extracellular domain connected through a seven transmembrane domain motif to the intracellular C-terminal tail1,2. The different mGluR subtypes are localized at both presynaptic and postsynaptic membranes, and are involved in the generation of slow excitatory and inhibitory synaptic potentials, modulation of synaptic transmission, synaptic integration, and plasticity3. mGluR6 belongs to the third group of metabotrophic glutamate receptors. Its expression in the central nervous system is restricted to retinal rod bipolar cells, and it’s localized to their postsynaptic dendritic membranes3,4. In the peripheral nervous system, it is also detected in bone marrow stromal cells6. mGluR6 inhibits adenylate cyclase, and was shown to inhibit Ca2+ influx and nitric oxide synthase activity3,5,6. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat mGluR6. Anti-mGluR6 (extracellular) antibody (#AG1248) can be used in western blot analysis, and has been designed to recognize mGluR6 from rat, mouse and human samples.
1. Con, P.J. et al. (2005) Nat. Rev. Neurosci. 6, 787.
2. Costantino, G. (2006) Curr. Pharm. Des. 12, 2159.
3. Ferraguti, F. et al. (2006) Cell Tissue Res. 326, 483.
4. Nakajima, Y. et al. (1993) J. Biol. Chem. 268, 11868.
5. Nawy, S. (2000) J. Neurosci. 20, 4471.
6. Foreman, M.A. et al. (2005) J. Cell Physiol. 204, 704.
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