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Excitatory Amino Acid Transporter 1 (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - Excitatory Amino Acid Transporter 1 (extracellular) Antibody AG1252-025
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: 1, 2. Anti-Excitatory Amino Acid Transporter 1 (extracellular) antibody (#AG1252) (1:500).
    3, 4. Anti-Excitatory Amino Acid Transporter 1 (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - Excitatory Amino Acid Transporter 1 (extracellular) Antibody AG1252-025
    Expression of EAAT1 in rat hippocampus
    Immunohistochemical staining was performed in perfusion-fixed free floating rat brain sections (frozen) using Anti-Excitatory Amino Acid Transporter 1 (extracellular) antibody (#AG1252), (1:100). A. EAAT1 staining (green) is particularly intense along the subgranular layer (arrows). B. Staining with mouse anti glial fibrillary acidic protein (red). C. Merged picture confirms presence of densely packed astrocytes along the subgranular layer. DAPI is used as a general cell marker (blue) .
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P24942
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 59697 Da
Homology Mouse - identical; human 12/13 amino acid residues identical.
Additional Information
Gene ID 29483
Other Names Excitatory amino acid transporter 1, Glial glutamate transporter, Sodium-dependent glutamate/aspartate transporter 1, GLAST-1, Solute carrier family 1 member 3, Slc1a3, Eaat1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)KQFKTSYEKRSFK, corresponding to amino acids 188-200 of rat EAAT1 (Accession P24942). 2nd extracellularֲ loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.4 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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L-Glutamate (Glu) is an abundant amino acid that functions as the major excitatory neurotransmitter in the central nervous system. However, excess of Glu in the extracellular synaptic milieu leads to neuronal cell death by a process known as excitotoxicity. The extracellular levels of Glu are regulated by a family of high affinity plasma membrane transporters called excitatory amino acid transporters (EAATs) which are responsible for re-uptake of Glu into the cells.1,2   The EAAT family includes five members (EAAT1-EAAT5) that are members of the solute carrier family 1 (SLC1) of sodium-dependent transporters that also includes the neutral amino acid transporters ASCT1 and ASCT2.   The Glu transporters present an unusual topology of eight transmembrane domains with two re-entrant loops and intracellular N- and C- termini. The transporter is likely assembled as a trimer where each monomer is a functional unit capable of binding the Glu substrate.   The transport of Glu into the cells by the EAAT transporters is coupled to the Na+ and K+ electrochemical gradient as a driving force. Hence, the uptake of Glu is dependent on the co-transport of three Na+ and one H+ ions, and the counter transport of one K+ ion.   In addition, to the well documented Glu uptake, the EAAT transporters show a Glu-independent Cl- conductance. The physiological significance of the Cl- current through the EAATs is currently unknown.1,2   EAAT1 as well as EAAT2, is expressed predominantly in glia cells (hence its original name: glial glutamate transporter or GLAST), while EAAT3, EAAT4 and EAAT5 are mostly expressed in neurons.   As mentioned earlier, EAAT transporters represent the only (significant) mechanism for removal of glutamate from the extracellular fluid and hence are essential for the long-term maintenance of low and non-toxic concentrations of glutamate and the preservation of normal excitatory synaptic transmission. In addition, to Glu uptake the glutamate transporters provide glutamate for the synthesis of γ-Aminobutyric acid (GABA), glutathione and protein, suggesting an interactive role between EAATs and cellular metabolism.1,2   Dysregulation of EAATs activities has been implicated in several neurodegenerative disorders such as Alzheimer’s disease, traumatic brain injury, epilepsy and schizophrenia, suggesting that EAATs can be a useful target for the treatment of these conditions.1,2   Abgent is pleased to offer a highly specific antibody directed against an epitope located at the extracellular 2nd loop of the rat EAAT1 receptor. Anti-Excitatory Amino Acid Transporter 1 (extracellular) antibody (#AG1252) can be used in Western blot and immunohistochemical applications, and recognizes EAAT1 from rat and mouse samples.


References 1. Tzingounis, A.V. and Wadiche, J.I. (2007) Nat. Rev. Neurosci. 8, 935. 2. Beart, P.M. and O’Shea, R.D. (2007) Br. J. Pharmacol. 150, 5.

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$ 375.00
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$ 575.00
Cat# AG1252-025
(40 western blots)
Availability: 2-3 weeks
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