|Application ||WB, IHC, FC, ICC, LCI|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||102232 Da|
|Homology||Mouse, human - identical.|
|Other Names||Metabotropic glutamate receptor 7, mGluR7, Grm7, Gprc1g, Mglur7|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide TISGSKKEDTDRKC, corresponding to amino acid residues 377-390 of rat mGluR7 (Accession P35400). Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by mass-spectroscopy and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Metabotropic glutamate receptors (mGluRs) belong to the super family of G-protein coupled receptors (seven transmembrane proteins). mGluRs are further divided into subfamilies: group I mGluRs (mGluR1 and mGluR5) which couple to Gq, thereby activating phospholipase C (PLC). Group II which include mGluR2 and mGluR3 couple to Gi, therefore inhibit the formation of adenylate cyclase. mGluR4, 6, 7, 8 which belong to group III also inhibit adenylate cyclase formation by coupling to Gi1. The C-terminus of these receptors has important functions in modulating their activity. This region is important for G-protein coupling, post-translational modifications like phosphorylation as well as protein-protein interactions. The C-terminal region is also subject to alternative splicing2. Indeed, mGluR7 can be detected as five different splice variants3. Like all group III metabotropic glutamate receptors, mGluR7 is localized presynaptically. It is highly expressed in the central nervous system and could also be detected in some peripheral organs like hair cells and spiral ganglion cells of the inner ear3. As opposed to other group III metabotropic glutamate receptors, mGluR7 has very low affinity for its substrate L-glutamate, therefore, only under high synaptic activity is the receptor active. This enables the receptor to act as a negative feedback loop by inhibiting the release of L-glutamate3,4. mGluR7 may play a molecular role in the pathways leading to emotion and cognition4. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat mGluR7. Anti-mGluR7 (extracellular) antibody (#AG1255) can be used in western blot analysis, immunocytochemistry and indirect flow cytometry applications. It has been designed to recognize mGluR7 from human, rat and mouse samples.
1. Kim, C.H. et al. (2008) J. Neurosci. Res. 86, 1.
2. Niswender, C.M. and Conn, P.J. (2010) Annu. Rev. Pharmacol. Toxicol. 50, 295.
3. Nicoletti, F. et al. (2011) Neuropharmacology 60, 1017.
4. O’Connor, R.M. et al. (2010) Eur. J. Pharmacol. 639, 123.
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