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mGluR4 (extracellular) Antibody

Affinity purified rabbit polyclonal antibody

  • WB - mGluR4 (extracellular) Antibody AG1256-050
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: 1, 2. Anti-mGluR4 (extracellular) antibody (#AG1256), (1:200). 3, 4. Anti-mGluR4 (extracellular) antibody, preincubated with the control peptide antigen.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P31423
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 101819 Da
Homology Mouse, human - identical.
Additional Information
Gene ID 24417
Other Names Metabotropic glutamate receptor 4, mGluR4, Grm4, Gprc1d, Mglur4
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide KPKGHPHMNSIR(C), corresponding to amino acid residues 33-44 of rat mGluR4 (Accession P31423). Extracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectroscopy and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 ml or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Citations (0)

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Metabotropic glutamate receptors (mGluRs) belong to the super family of G-protein coupled receptors (seven transmembrane proteins). mGluRs are further divided into subfamilies: group I mGluRs (mGluR1 and mGluR5) which couple to Gq, thereby activating phospholipase C (PLC). Group II which include mGluR2 and mGluR3 couple to Gi, therefore inhibit the formation of adenylate cyclase. mGluR4, 6, 7, 8 which belong to group III also inhibit adenylate cyclase formation by coupling to Gi1. The C-terminus of these receptors has important functions in modulating their activity. This region is important for G-protein coupling, post-translational modifications like phosphorylation as well as protein-protein interactions. The C-terminal region is also subject to alternative splicing2. mGluR4 is predominantly expressed presynaptically in neurons and in the cerebellum. A splice variant of the protein is expressed in taste buds. This variant lacks a large portion of the N-terminus and is normally referred to taste mGluR43,4. It is responsible (along with taste mGluR1) for mediating the taste of monosodium glutamate (or unami)2,4. mGluR4 knock out mice display impaired cerebellar synaptic plasticity as well as some learning disabilities5. In addition, this receptor has emerged as a target for the treatment of Parkinson’s disease6. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat mGluR4. Anti-mGluR4 (extracellular) antibody (#AG1256) can be used in western blot analysis, and has been designed to recognize mGluR4 from rat, mouse and human samples.


1. Kim, C.H. et al. (2008) J. Neurosci. Res. 86, 1.
2. Niswender, C.M. and Conn, P.J. (2010) Annu. Rev. Pharmacol. Toxicol. 50, 295.
3. Chaudhari, N. et al. (2000) Nat. Neurosci. 3, 113.
4. Chaudhari, N. et al. (2009) Am. J. Clin. Nutr. 90, 738S.
5. Pekheltski, R. et al. (1996) J. Neurosci. 16, 6364.
6. Conn, P.J. et al. (2005) Nat. Rev. Neurosci. 6, 787.

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$ 475.00
$ 575.00
Cat# AG1256-050
(40 western blots)
Availability: 2-3 weeks
Bulk Size
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