|Reactivity||Human, Mouse, Rat|
|Calculated MW||101819 Da|
|Homology||Mouse, human - identical.|
|Other Names||Metabotropic glutamate receptor 4, mGluR4, Grm4, Gprc1d, Mglur4|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide KPKGHPHMNSIR(C), corresponding to amino acid residues 33-44 of rat mGluR4 (Accession P31423). Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by mass-spectroscopy and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 ml or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Metabotropic glutamate receptors (mGluRs) belong to the super family of G-protein coupled receptors (seven transmembrane proteins). mGluRs are further divided into subfamilies: group I mGluRs (mGluR1 and mGluR5) which couple to Gq, thereby activating phospholipase C (PLC). Group II which include mGluR2 and mGluR3 couple to Gi, therefore inhibit the formation of adenylate cyclase. mGluR4, 6, 7, 8 which belong to group III also inhibit adenylate cyclase formation by coupling to Gi1. The C-terminus of these receptors has important functions in modulating their activity. This region is important for G-protein coupling, post-translational modifications like phosphorylation as well as protein-protein interactions. The C-terminal region is also subject to alternative splicing2. mGluR4 is predominantly expressed presynaptically in neurons and in the cerebellum. A splice variant of the protein is expressed in taste buds. This variant lacks a large portion of the N-terminus and is normally referred to taste mGluR43,4. It is responsible (along with taste mGluR1) for mediating the taste of monosodium glutamate (or unami)2,4. mGluR4 knock out mice display impaired cerebellar synaptic plasticity as well as some learning disabilities5. In addition, this receptor has emerged as a target for the treatment of Parkinson’s disease6. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat mGluR4. Anti-mGluR4 (extracellular) antibody (#AG1256) can be used in western blot analysis, and has been designed to recognize mGluR4 from rat, mouse and human samples.
1. Kim, C.H. et al. (2008) J. Neurosci. Res. 86, 1.
2. Niswender, C.M. and Conn, P.J. (2010) Annu. Rev. Pharmacol. Toxicol. 50, 295.
3. Chaudhari, N. et al. (2000) Nat. Neurosci. 3, 113.
4. Chaudhari, N. et al. (2009) Am. J. Clin. Nutr. 90, 738S.
5. Pekheltski, R. et al. (1996) J. Neurosci. 16, 6364.
6. Conn, P.J. et al. (2005) Nat. Rev. Neurosci. 6, 787.
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