|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||95774 Da|
|Homology||Mouse, human, dog, monkey - identical.|
|Other Names||Metabotropic glutamate receptor 2, mGluR2, Grm2, Gprc1b, Mglur2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide SLSRGADGSRHIC, corresponding to amino acids 109-121 of rat mGluR2 (Accession P31421). Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
L-Glutamate, the major excitatory neurotransmitter in the central nervous system, operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors). The metabotropic glutamate receptors family includes eight members (mGluR1-8) that have been divided into three groups based on their sequence homology, pharmacology and signal transduction. Group II of the metabotropic glutamate receptors includes the mGluR2 and mGluR3 receptors. The receptors present the typical G-protein coupled receptor (GPCR) signature topology: seven transmembrane domains with a large extracellular N-terminus domain that contains the glutamate binding site, and an intracellular C-terminus one.1,2 mGluR2 and mGluR3 are coupled to Gi/Go and hence inhibit cAMP formation following receptor activation.1, 2 mGluR2 is widely distributed throughout the brain with high expression in several limbic areas including the cortex, hippocampus and amygdala. mGluR2 is localized primarily presinaptically, although postsynaptical localization has also been described. In line with its presynaptical localization, mGluR2 is thought to function as an autoreceptor in a negative feedback mechanism that suppress further release of glutamate from the cell on which it is expressed. The involvement of mGluR2 in neuronal excitability and synaptic transmission suggests that modulation of this receptor is a promising strategy for the treatment of neurological and neuropsychiatric disorders such as anxiety, schizophrenia, and pain.3,4 Abgent is pleased to offer a highly specific antibody directed against an epitope located at the extracellular N-terminus domain of the rat mGluR2 receptor. The epitope is specific for mGluR2 and will not cross-react with the closely related mGluR3 channel. Anti- mGluR2 (extracellular) antibody (#AG1258) can be used in Western blot analysis and immunohistochemical applications, and recognizes mGluR2 from rat and mouse samples.
1. Flor, P.J. et al. (1995) Eur. J. Neurosci., 7, 622.
2. Conn, P.J. and Pin, J.P. (1997) Annu Rev Pharmacol Toxicol 37, 205.
3. Swanson, C.J. et al. (2005) Nat. Rev. Drug Discov. 4, 131.
4. Swanson, C.J. et al. (2005) Nat. Rev. Drug Discov. 4, 131.
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