|Application ||WB, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||100373 Da|
|Homology||Mouse, human, dog - identical.|
|Other Names||Glutamate receptor 3, GluR-3, AMPA-selective glutamate receptor 3, GluR-C, GluR-K3, Glutamate receptor ionotropic, AMPA 3, GluA3, Gria3, Glur3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)EKPFHLNYHVDHLD, corresponding to amino acid residues 60-73 of rat AMPA Receptor 3 (Accession P19492 ).ֲ ֲ Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.6 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
L-Glutamate, the major excitatory neurotransmitter in the central nervous system, operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors). The ligand-gated ion channel family consists of 15 members that have been subdivided into three families based on their pharmacological profile: the a-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid (AMPA) preferring receptors, the N-methyl-D-aspartate (NMDA) preferring and the kainate preferring receptors. The AMPA receptor subfamily includes four members AMPA1 to AMPA4, also known as GluR1 to GluR4 respectively. The functional AMPA channel is believed to be a tetramer, with most neuronal AMPA receptors being heterotetramers composed of AMPA1 plus AMPA2 or AMPA2 plus AMPA3 channels, although homotetramers can also been found. AMPA receptors are permeable to cations Na+, K+ and Ca2+. The Ca2+ permeability is dependent on the presence of AMPA2: whenever this subunit is present, the channel will be impermeable to Ca2+.1 Gating of AMPA receptors by glutamate is extremely fast and therefore the AMPA receptors mediate most excitatory (depolarizing) currents in the brain during basal neuronal activity. The depolarization caused by the activation of post-synaptic AMPA receptors is necessary for the activation of NMDA receptors that will open only in the presence of both glutamate and a depolarized membrane potential. Synaptic strength that is defined as the level of post-synaptic depolarization can be long term (hence the term long term potentiation, LTP) and therefore induce changes in signaling and protein synthesis in the activated neuron. These changes are associated with memory formation and learning. Changes in synaptic strength are thought to involve rapid movement of the AMPA receptors in and out of the synapses and a great deal of effort has focused in understanding the mechanisms that govern AMPA receptor trafficking.2 The exact physiological role of the AMPA3 receptor is not clear but a role in the modulation of oscillatory networks affecting sleep and breathing has been suggested.3 Abgent is pleased to offer a highly specific antibody directed against a well conserved epitope located in the extracellular N-terminal region of the rat AMPA Receptor 3. The antibody does not cross react with the related AMPA Receptor 2 subunit. Anti-AMPA Receptor 3 (GluA3) (extracellular) (#AG1259) antibody can be used in western blot, immunohistochemical, and immunoprecipitation applications and will recognize AMPA Receptor 3 from rat, mouse, human and dog samples.
References 1. Dingledine, R. et al. (1999) Pharmacol. Rev. 51, 7. 2. Lynch, M.A. (2004) Physiol. Rev. 84, 87. 3. Steenland, H.W. et al. (2008) Eur. J. Neurosci. 27, 1166.
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