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>   home   >   Products   >   Primary Antibodies   >   Kainate Receptor GluK1 (extracellular) Antibody   

Kainate Receptor GluK1 (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - Kainate Receptor GluK1 (extracellular)  Antibody AG1261-025
    Western blot analysis of rat brain membranes:
    1. Anti-Kainate Receptor GluK1 (extracellular) antibody (#AG1261), (1:200).
    2. Anti-Kainate Receptor GluK1 (extracellular) antibody, preincubated with the control peptide antigen.
  • ICC - Kainate Receptor GluK1 (extracellular)  Antibody AG1261-025
    Expression of Kainate Receptor GluK1 in rat DRG neurons Immunocytochemical staining of living rat dorsal root ganglion (DRG). A. Extracellular staining of cells using Anti-Kainate Receptor GluK1 (extracellular) antibody (#AG1261), (1:50) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (red). B. Live view of the same field.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P22756
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 107840 Da
Homology Human, dog, monkey - 15/16 amino acid residues identical.
Additional Information
Gene ID 29559
Other Names Glutamate receptor ionotropic, kainate 1, GluK1, Glutamate receptor 5, GluR-5, GluR5, Grik1, Glur5
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)KASGEVSKHLYKVWKK, corresponding to amino acid residuesֲ 402-417 of ratֲ Kainate Receptor GluR5ֲ (Accession P22756). Extracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
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L-Glutamate, the major excitatory neurotransmitter in the central nervous system (CNS), operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein-coupled receptors).   The ligand-gated ion channel family consists of fifteen members that have been subdivided into three families based upon their pharmacological profile: the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-preferring receptors, the N-methyl-D-aspartate (NMDA)-preferring receptors, and the kainate-preferring receptors.   The kainate receptor subfamily consists of five members that have been further subdivided into two classes based upon structural homology and functional characteristics. GluR5, GluR6, and GluR7receptor subunits share a high degree of homology and are able to form functional channels when expressed in heterologous systems. The KA-1 and KA-2 receptors are unable to form functional channels on their own, but when coexpressed with GluR5-7 receptor subunits, they form channels with high affinity for kainate.1,2   Like AMPA receptors, the functional unit of endogenous kainate receptors is believed to be a tetramer, which can be either homomeric or heteromeric. Kainate receptors GluR5 and GluR6 (but not GluR7, KA-1, or KA-2) can undergo RNA editing; as in the AMPA receptor GluR2, a glutamine (Q) residue in the channel pore is edited to encode arginine (R) in the mature protein. Substitution of Q with R modulates the properties of the channel, producing channels with reduced single channel conductance and lower permeability to Ca2+.1,2   GluR5 is highly expressed in dorsal root ganglion (DRG) neurons of the peripheral nervous system (PNS) as well as in several structures of the CNS including the amygdala, the hipoccampus, and Purkinje cells of the cerebellum.   GluR5 has been implicated in the modulation of synaptic transmission and synaptic plasticity in both the CNS and PNS, but the exact physiological function of GluR5 is not well understood. Nevertheless, an involvement in persistent pain and some types of learning has been proposed. Abgent is pleased to offer Anti-Kainate Receptor GluK1 (extracellular) antibody (#AG1261), a new highly specific antibody directed against the extracellular N-terminus domain of the rat kainate receptor GluK1 channel. The antibody can be used in western blot and in immunofluorescence applications.


References 1. Lerma, J. et al. (2001) Physiol. Rev. 81, 971. 2. Dingledine, R. et al. (1999) Pharmacol. Rev. 51, 7.

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Cat# AG1261-025
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