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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   mGluR5 (extracellular) Antibody   

mGluR5 (extracellular) Antibody

Affinity purified rabbit polyclonal antibody

  • WB - mGluR5 (extracellular) Antibody AG1262-025
    Western blot analysis of rat brain membranes: 1. Anti-mGluR5 (extracellular) antibody (#AG1262), (1:500). 2. Anti-mGluR5 (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - mGluR5 (extracellular) Antibody AG1262-025
    Expression of mGluR5 in rat cerebellum and hippocampus Immunohistochemical staining of perfusion-fixed frozen rat cerebellum sections using Anti-mGluR5 (extracellular) antibody (#AG1262), (1:50). mGluR5 (red) was detected in cerebellar Purkinje cells (vertical arrows) and in the molecular layer (horizontal arrows). Staining with mouse anti-parvalbumin (green) revealed co-localization in Purkinje but not in the molecular layer. Little staining of mGluR5 was detected in the granule layer. DAPI counterstain is used to visualize nuclei of all cells (blue).
  • IHC - mGluR5 (extracellular) Antibody AG1262-025
    Expression of mGluR5 in rat cerebellum and hippocampus Immunohistochemical staining of perfusion-fixed frozen rat hippocampus sections using Anti-mGluR5 (extracellular) antibody (#AG1262), (1:50). mGluR5 (red) was detected in CA3 cells (arrows). Staining with mouse anti-parvalbumin (green) revealed co-localization in pyramidal layer. DAPI counterstain was used to visualize nuclei of all cells (blue)
  • ICC - mGluR5 (extracellular) Antibody AG1262-025
    Expression of mGluR5 in rat GH3 pituitary cells Immunocytochemical staining of live intact rat GH3 pituitary cells using Anti-mGluR5 (extracellular) antibody (#AG1262), (1:100), followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P31424
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 131885 Da
Homology Mouse - identical; human - 13/14 amino residues identical.
Additional Information
Gene ID 24418
Other Names Metabotropic glutamate receptor 5, mGluR5, Grm5, Gprc1e, Mglur5
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide EGFAQENSKYNKTC, corresponding to amino acids 367-380 of rat mGluR5 (Accession P31424). Extracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.75 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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L-Glutamate, the major excitatory neurotransmitter in the central nervous system, operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors). The metabotropic glutamate receptors family includes eight members (mGluR1-8) that have been divided into three groups based on their sequence homology, pharmacology and signal transduction. Group I of the metabotropic glutamate receptors includes the mGluR1 and mGluR5 receptors. The receptors present the typical G-protein coupled receptor (GPCR) signature topology: seven transmembrane domains with a large extracellular N-terminus domain and an intracellular C-terminus one. The N-terminus domain of group I receptors contains the glutamate binding site while the cytoplasmic C-terminus domain has an important role in the regulation of receptor activity through interactions with other proteins such as the Homer adaptor proteins. mGluR1 and mGluR5 receptors signal through Gq/G11 that activates phospholipase C and ultimately produces an increase in inositol trisphosphate and cytosolic Ca2+. More downstream signaling pathways include activation of PKC and modulation of Ca2+ and K+ ion channels. Activation of signaling pathways independent of G-proteins has also been reported. mGluR5 is predominantly expressed in nervous tissue although expression in several non-neural cell types has also been described. In the brain it is highly expressed in the cortex, basal ganglia and hippocampus. The mGluR5 receptor is involved in several physiological processes such as neuronal development, induction of long-term potentiation (LTP) and depression (LTD) as well as in pathological disorders such as brain trauma, chronic pain, Parkinson’s and Huntington’s disease. Abgent is pleased to offer a highly specific antibody directed against the extracelluar N-terminus domain of the rat mGluR5 receptor. Anti-mGluR5 (extracellular) antibody (#AG1262) can be used in Western blot analysis, immunhistochemical and immunocytochemical applications, and recognizes mGluR5 in rat samples.


1. Kew, J.N.C. and Kemp, J.A. (2005) Psychopharmacol.(Berl.) 179, 4.
2. Ferraguti, F. and Shigemoto, R. (2006) Cell Tissue Res. 326, 428.
3. Dhami, G.K. and Ferguson, S.S.G. (2006) Pharmacol. Ther. 111, 260.

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$ 375.00
$ 475.00
$ 575.00
Cat# AG1262-025
(40 western blots)
Availability: 2-3 weeks
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