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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Amyotrophic Lateral Sclerosis (ALS)   >   NMDA Receptor 2A (NR2A) (extracellular) Antibody   

NMDA Receptor 2A (NR2A) (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - NMDA Receptor 2A (NR2A) (extracellular) Antibody AG1266-025
    Western blot analysis of rat brain lysates:
    1. Anti-NMDA Receptor 2A (GluN2A) (extracellular) antibody (#AG1266), (1:600).
    2. Anti-NMDA Receptor 2A (GluN2A) (extracellular) antibody, preincubated with the control peptide antigen.
  • IP - NMDA Receptor 2A (NR2A) (extracellular) Antibody AG1266-025
    Immunoprecipitation of rat brain lysates
    1. Cell lysate.
    2. Cell lysates + protein A beads + Anti-NMDA Receptor 2A (GluN2A) (extracellular) antibody (#AG1266).
    3. Cell lysates + protein A beads + pre-immune rabbit serum. 

    Black arrow indicates the NMDA Receptor 2A protein while the red arrow shows the IgG heavy chain. Immunoblot was performed with Anti-NMDA Receptor 2A (GluN2A) (extracellular) antibody.
  • IHC - NMDA Receptor 2A (NR2A) (extracellular) Antibody AG1266-025
    Immuno-colocalization of GluN2A and GluN2B in mouse deep cerebellar nucleus Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-NMDA Receptor 2B (GluN2B) (extracellular)-ATTO-594 antibody (#AGC-003-AR), (1:60) and Anti-NMDA Receptor 2A (GluN2A) (extracellular) antibody (#AG1266), (1:200). A. Sections were incubated with Anti-NMDA Receptor 2A (GluN2A) (extracellular) antibody, followed by goat anti-rabbit-Alexa-488 (green). B. The same sections were incubated with Anti-NMDA Receptor 2B (GluN2B) (extracellular)-ATTO-594 antibody (red). C. Merge of A and B demonstrates the ubiquitous colocalization of the GluN2A and GluN2B subunits in cells with neuronal profiles in this nucleus.  Arrows point at an example of NR2A and NR2B co-expression.
  • IHC - NMDA Receptor 2A (NR2A) (extracellular) Antibody AG1266-025
    Expression of NMDA Receptor 2A in rat hippocampus Immunohistochemical staining of  rat hippocampal dentate gyrus with Anti-NMDA Receptor 2A (GluN2A) (extracellular) antibody (#AG1266). A. NMDA Receptor 2A (green) appears diffusely in the outer molecular layer of the dentate gyrus (Out Mol.) and in cells along the subgranular layer (arrows). B. Staining of parvalbumin (PV, red) identifies interneurons in the dentate gyrus. C. Confocal merge demonstrates localization of PV in some neurons with NMDA Receptor 2A.
  • ICC - NMDA Receptor 2A (NR2A) (extracellular) Antibody AG1266-025
    Expression of NMDA Receptor 2A in rat C6 glioma cells Immunocytochemical staining of live intact rat C6 glioma cells.
    A. Cells were stained with Anti-NMDA Receptor 2A (GluN2A) (extracellular) antibody (#AG1266), (1:100), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (red). Cell nuclei were stained with the cell permeable dye Hoechst 33342 (blue staining). B. Live view of the same field.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q00959
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 165469 Da
Homology Human, mouse - identical.
Additional Information
Gene ID 24409
Other Names Glutamate receptor ionotropic, NMDA 2A, GluN2A, Glutamate [NMDA] receptor subunit epsilon-1, N-methyl D-aspartate receptor subtype 2A, NMDAR2A, NR2A, Grin2a
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide GHSHDVTERELRN(C), corresponding to amino acid residues 41-53 of rat NMDA Receptor 2A (Accession Q00959). Extracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.55 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
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The NMDA receptors are members of the glutamate receptor family of ion channels that also include the AMPA and Kainate receptors.   The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functional NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit.   The NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg2+. Neuronal depolarization relieves the Mg2+ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca2+ influx differing from the other glutamate receptor ion channels that are non-selective cation channels.   Ca2+ entry through the NMDAR regulates numerous downstream signaling pathways including long term potentiation (a molecular model of memory) and synaptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinson’s and Alzheimer’s disease.   NMDA receptors expression and function are modulated by a variety of factors including receptor trafficking to the synapses and internalization as well as phosphorylation and interaction with other intracellular proteins.


References 1. Dingledine, R. et al. (1999) Pharmacol. Rev. 51, 7. 2. Mayer, M.L. and Armstrong, N. (2004) Annu. Rev. Physiol. 66, 161. 3. Prybylowski, K. and Wenthold, R.J. (2004) J. Biol. Chem. 279, 9673. 4. Mayer, M.L. (2006) Nature 440, 456.

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Cat# AG1266-025
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