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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Amyotrophic Lateral Sclerosis (ALS)   >   NMDA Receptor 1 (NR1) (extracellular) Antibody   

NMDA Receptor 1 (NR1) (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - NMDA Receptor 1 (NR1) (extracellular) Antibody AG1267-025
    Western blot analysis of rat brain lysate:
    1. Anti-NMDA Receptor 1 (GluN1) (extracellular) antibody (#AG1267), (1:600).
    2. Anti-NMDA Receptor 1 (GluN1) (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - NMDA Receptor 1 (NR1) (extracellular) Antibody AG1267-025
    Immuno-colocalization of GluN1 and CALHM1 in mouse hippocampal CA1 region Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-NMDA Receptor 1 (GluN1) (extracellular) antibody (#AG1267), (1:200) and Anti-Calcium Homeostasis Modulator 1-ATTO-594 antibody (#ACC-101-AR), (1:60). A. Sections were stained with Anti-NMDA Receptor 1 (GluN1) (extracellular) antibody, followed by goat-anti-rabbit-Cy2 (green). Staining reveals expression in neurons of the pyramidal layer (an arrow points at the layer). B. The same section was incubated with Anti-Calcium Homeostasis Modulator 1-ATTO-594 antibody, (red). C. Merge of the two images demonstrates colocalization of GluN1 and CALHM1 in pyramidal neurons.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P35439
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 105509 Da
Homology Human, mouse - identical.
Additional Information
Gene ID 24408
Other Names Glutamate receptor ionotropic, NMDA 1, GluN1, Glutamate [NMDA] receptor subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, NMD-R1, Grin1, Nmdar1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide ETEKPRGYQMSTRLK(C), corresponding to amino acid residuesֲ 385-399 of rat NMDA Receptor 1 (Accession P35439).ֲ ֲ  Extracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Application Details   Live cell imaging (LCI): - Rat live and intact hippocampal neurons (1:300), (see Mikasova, L. et al. (2012) in Product Citations).
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Research Areas
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The NMDA receptors are members of the glutamate receptor family of ion channels that also include the AMPA and Kainate receptors. The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functional NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit. The NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg2+. Neuronal depolarization relieves the Mg2+ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca2+ influx differing from the other glutamate receptor ion channels that are non-selective cation channels. Ca2+ entry through the NMDAR regulates numerous downstream signaling pathways including long term potentiation (a molecular model of memory) and synaptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinson’s and Alzheimer’s disease. NMDA receptors expression and function are modulated by a variety of factors including receptor trafficking to the synapses and internalization as well as phosphorylation and interaction with other intracellular proteins.


References 1. Dingledine, R. et al. (1999) Pharmacol. Rev. 51, 7. 2. Mayer, M.L. and Armstrong, N. (2004) Annu. Rev. Physiol. 66, 161. 3. Prybylowski, K. and Wenthold, R.J. (2004) J. Biol. Chem. 279, 9673 4. Mayer, M.L. (2006) Nature 440, 456.

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$ 375.00
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$ 575.00
Cat# AG1267-025
(40 western blots)
Availability: 2-3 weeks
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