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GABA (B) R2 Antibody

Affinity purified rabbit polyclonal antibody

  • WB - GABA (B) R2 Antibody AG1268-050
    Western blot analysis of lysate from human brain tissue lysate, using GABBR2 Antibody(Cat. #AG1268). AG1268 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
  • WB - GABA (B) R2 Antibody AG1268-050
    Western blot analysis of rat brain membrane: 1. Anti-GABA(B) R2 antibody (#AG1268), (1:200). 2. Anti-GABA(B) R2 antibody preincubated with the control peptide antigen.
  • IHC - GABA (B) R2 Antibody AG1268-050
    Expression of GABA(B) R2 in mouse hippocampus Immunohistochemical staining of mouse hippocampus frozen sections using Anti-GABA(B) R2 antibody (#AG1268 ), (1:100). A. GABA(B) R2 staining (green) is detected in layers including the stratum lacunosum moleculare (SLM) and stratum radiatum of the CA3 layer (star). B. Staining with mouse anti-GAP43 antibody (red) sets apart the SLM. C. Confocal merge suggests the presence of GABA(B) R2 in layers, but not in pyramidal neurons.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75899
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 105821 Da
Homology Rat, mouse, bovine - identical.
Additional Information
Gene ID 9568
Other Names Gamma-aminobutyric acid type B receptor subunit 2, GABA-B receptor 2, GABA-B-R2, GABA-BR2, GABABR2, Gb2, G-protein coupled receptor 51, HG20, GABBR2, GPR51, GPRC3B
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide CKDPIEDINSPEHIQRRLSL, corresponding to amino acid residues 875-894 of human GABA(B) R2 (Accession number O75899). Intracellular, C-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Application Details Western blot analysis (WB): - HEK-293 cells stably expressing human GABA(B) R2 (see Han, C. et al. (2013) in Product Citations).
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.85 mg/ml.
Buffer After Reconstitution
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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GABA (gamma-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system and plays a crucial role in the modulation of neuronal activity.1,2 The GABA transmitter interacts with three types of receptors: the ionotropic receptors, GABA (A) R and GABA (C) R, and the metabotropic GABA (B) receptor, [GABA (B) R].3 The latter belongs to the G-protein-coupled receptor (GPCR) superfamily and mediates slow synaptic inhibition in the brain and spinal cord1,4. The functional GABA (B) receptor [GABA (B)R] is a heterodimer consisting of two subunits, the GABA (B) R1 and the GABA (B) R2. These subunits were demonstrated to have complementary roles essential for the functional receptor.5,6 GABA (B) R2 has been shown to be essential for trafficking and for G-protein binding while the GABA (B) R1 subunit has been demonstrated to be important for agonist and antagonist binding. GABA (B) R2 is highly expressed in brain, especially in the cerebral cortex, thalamus, and hippocampus, spinal cord, and, to a lesser extent, in testis.7


1. Bowery, N.G. and Smart, T.G. (2006) Br. J. Pharmacol. 147, S109.
2. Bettler, B. et al. (2004) Physiol. Rev. 84, 835.
3. Boller, M. and Schmidt, M. (2003) J. Neurophysiol. 89, 2035.
4. Robbins, M.J. et al. (2001) J. Neurosci. 21, 8043.
5. Margeta-Mitrovic M. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 14649.
6. Margeta-Mitrovic M. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 14643.

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$ 475.00
$ 575.00
Cat# AG1268-050
(40 western blots)
Availability: 2-3 weeks
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