|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||105821 Da|
|Homology||Rat, mouse, bovine - identical.|
|Other Names||Gamma-aminobutyric acid type B receptor subunit 2, GABA-B receptor 2, GABA-B-R2, GABA-BR2, GABABR2, Gb2, G-protein coupled receptor 51, HG20, GABBR2, GPR51, GPRC3B|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide CKDPIEDINSPEHIQRRLSL, corresponding to amino acid residues 875-894 of human GABA(B) R2 (Accession number O75899). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Application Details||Western blot analysis (WB): - HEK-293 cells stably expressing human GABA(B) R2 (see Han, C. et al. (2013) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.85 mg/ml.|
|Buffer After Reconstitution|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
GABA (gamma-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system and plays a crucial role in the modulation of neuronal activity.1,2 The GABA transmitter interacts with three types of receptors: the ionotropic receptors, GABA (A) R and GABA (C) R, and the metabotropic GABA (B) receptor, [GABA (B) R].3 The latter belongs to the G-protein-coupled receptor (GPCR) superfamily and mediates slow synaptic inhibition in the brain and spinal cord1,4. The functional GABA (B) receptor [GABA (B)R] is a heterodimer consisting of two subunits, the GABA (B) R1 and the GABA (B) R2. These subunits were demonstrated to have complementary roles essential for the functional receptor.5,6 GABA (B) R2 has been shown to be essential for trafficking and for G-protein binding while the GABA (B) R1 subunit has been demonstrated to be important for agonist and antagonist binding. GABA (B) R2 is highly expressed in brain, especially in the cerebral cortex, thalamus, and hippocampus, spinal cord, and, to a lesser extent, in testis.7
1. Bowery, N.G. and Smart, T.G. (2006) Br. J. Pharmacol. 147, S109.
2. Bettler, B. et al. (2004) Physiol. Rev. 84, 835.
3. Boller, M. and Schmidt, M. (2003) J. Neurophysiol. 89, 2035.
4. Robbins, M.J. et al. (2001) J. Neurosci. 21, 8043.
5. Margeta-Mitrovic M. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 14649.
6. Margeta-Mitrovic M. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 14643.
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