|Reactivity||Human, Mouse, Rat|
|Calculated MW||111534 Da|
|Homology||Mouse, human, canine, bovine - identical.|
|Other Names||Gamma-aminobutyric acid type B receptor subunit 1, GABA-B receptor 1, GABA-B-R1, GABA-BR1, GABABR1, Gb1, Gabbr1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide CRGEREVVGPKVRK, corresponding to amino acid residues 66-79 of rat GABA(B) R1 (Accession Q9Z0U4).ֲ ֲ Extracellular, N-terminus.Does not recognize alternative splice 1b.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
GABA (gamma-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system and plays a crucial role in the modulation of neuronal activity.1,2 The GABA transmitter interacts with three types of receptors: the ionotropic receptors, GABA (A)R and GABA (C)R, and the metabotropic GABA (B) receptor, [GABA (B)R].3 The latter belongs to the G-protein-coupled receptor superfamily and mediates slow synaptic inhibition in the brain and spinal cord1,4. The functional GABA (B) receptor [GABA (B)R] is a heterodimer consisting of two subunits, the GABA (B)R1 and the GABA (B)R2. These subunits were demonstrated to have complementary roles essential for the functional receptor.5,6 The GABA (B)R1 subunit was demonstrated to be important for agonist and antagonist binding, while GABA (B)R2 was shown to be essential for trafficking and for G-protein binding. To date, eight alternatively spliced isoforms of GABA (B) R1 have been proposed. These are named 1a-1h, of which 1a and 1b are the most prominent. Only 1a, 1b, and 1c appear to act as functional subunits.6,7 They are widespread, being expressed in testis, stomach, spinal cord, and brain. Isoform 1b is also expressed in kidney and liver.8 Abgent is please to offer a highly specific antibody directed against an epitope located at the extracellular N-terminal domain of rat GABA (B) R1. Anti-GABA(B) R1 (extracellular) antibody (#AG1269) can be used in Western blot analysis and immunohistochemistry applications, and recognizes GABA(B) R1 in rat and mouse samples.
References 1. Bowery, N.G. and Smart, T.G. (2006) Br. J. Pharmacol. 147, S109. 2. Bettler, B. et al. (2004) Physiol. Rev. 84, 835. 3. Boller, M. and Schmidt, M. (2003) J. Neurophysiol. 89, 2035. 4. Robbins, M.J. et al. (2001) J. Neurosci. 21, 8043. 5. Margeta-Mitrovic M. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 14649. 6. Margeta-Mitrovic M. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 14643. 7. http://www.iuphar-db.org/DATABASE/FamilyMenuForward?familyId=26. 8. http://www.uniprot.org/uniprot/Q9Z0U4.
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