|Calculated MW||53551 Da|
|Homology||Mouse - 16/18 amino acid residues identical; human - 14/18 amino acid residues identical.|
|Other Names||Gamma-aminobutyric acid receptor subunit gamma-1, GABA(A) receptor subunit gamma-1, Gabrg1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)HAGSTLIPMNNISMPQGE, corresponding to amino acid residues 382-399 of rat GABA(A)־³1 Receptor (Accession P23574 ). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
The neurotransmitter GABA (γ-aminobutyric acid) inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells1. There are two major types of GABA receptors: the ionotropic GABAA (GABAA R) and the metabotropic GABAB receptors. GABAA R belongs to the ligand gated ion channel superfamily1,2. The binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance, resulting in inhibition of neural activity2,3. They are composed of heteropentamer, with all of the five subunits contributing to the pore formation. To date, eight subunit isoforms have been cloned: α, β, γ, δ, ε, π, θ, and ρ1. The native GABAA receptor, in most cases, consists of 2α, 2β and 1γ subunit. Three γ subunits genes have been identified in mammals. mRNA of the γ1 subunit is detected in the amygdala and septum4. Abgent is pleased to offer a highly specific antibody directed against an epitope of rat GABA(A) γ1 subunit. Anti-GABA(A) γ1 Receptor antibody (#AG1270) can be used in western blot analysis and was designed to recognize GABA(A) γ1 from human, rat and mouse samples.
References 1. Mihic, S.J. et al. (1994) J.Neurotransmit. Rev. 21, 127. 2. Whiting, P.J. et al. (1999) Neurochem. Int. 34, 379. 3. Ashcroft, F.M. (2000) Ion Channels and Disease Ed 1. 4. Mehta, A.K. and Ticku, M.K. (1999) Brain Res. Rev. 29, 196.
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