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Affinity purified polyclonal antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P28471 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 60951 Da |
| Homology | Mouse, rat - 13/14 amino acid residues identical; human - 10/14 amino acid residues identical. |
| Gene ID | 140675 |
|---|---|
| Other Names | Gamma-aminobutyric acid receptor subunit alpha-4, GABA(A) receptor subunit alpha-4, Gabra4 |
| Related products for control experiments | Control peptide antigen (supplied with the antibody free of charge). |
| Target/Specificity | Peptide NSKDEKLSPENFTR(C), corresponding to amino acid residues 37-50 of ֲ rat ֲ GABA(A) ־±4 with replacement of C44 with serine (Accession ֲ P28471 ). Extracellular, N-terminus. |
| Dilution | WB~~1:200-1:2000 |
| Peptide Confirmation | Confirmed by mass-spectrography and amino acid analysis. |
| Format | Affinity purified antibody, lyophilized powder |
| Reconstitution | 50 µl or 0.2 ml deionized water, depending on the sample size. |
| Antibody Concentration After Reconstitution | 0.8 mg/ml. |
| Buffer After Reconstitution | Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
| Storage Before Reconstitution | Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. |
| Storage After Reconstitution | The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min). |
| Control Antigen Storage Before Reconstitution | Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. |
| Control Antigen Reconstitution | 100 µl water. |
| Control Antigen Storage After Reconstitution | -20ºC. |
| Preadsorption Control | 1 µg peptide per 1 µg antibody. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake, and metabolism all occur in the nervous system1. The GABA transmitter interacts with two major types of receptors: ionotropic GABAA receptors (GABAAR) and metabotropic receptors (GABABR). GABAARs belong to the ligand-gated ion channel superfamily2. GABA inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells3. Binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance that results in inhibition of neural activity2. GABAARs are heteropentamers, in which all five subunits contribute to the pore formation. To date, eight subunit isoforms have been cloned: α, β, γ, δ, ε, π, θ, and ρ1. Six α subunit isoforms have been found to exist in mammals (α1-α6). In most cases, native GABAA receptors consist of 2α, 2β, and 1γ subunits. The α subunit is the most common and is expressed ubiquitously. It determines the affinity of GABAARs for allosteric ligands. Each subtype has a unique regional expression in the brain, and individual neurons often express multiple subtypes4. For example, the α4 subunit is detected in the hippocampus, cortex, olfactory bulb and in the basal forebrain5. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat GABA(A) α4 subunit. Anti-GABA(A) α4 Receptor (extracellular) antibody (#AG1272) can be used in western blot and immunohistochemistry applications. It was designed to recognize GABA(A) α4 from human, rat and mouse samples.
References
References 1. Owens, D.F. and Kriegstein, A.R. (2002) Nat. Rev. Neurosci. 3, 715. 2. Whiting, P.J. (1999) Neurochem. Int. 34, 387. 3. Mihic, S.J. and Harris, R.A. (1997) Alcohol Health Res. World 21, 127. 4. Neelands, T.R. et al. (1999) J. Neurosci. 19, 7057. 5. Olsen, R.W. and Tobbin, A.J. (1990) FASEBS J. 4, 1469.
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