|Calculated MW||54296 Da|
|Homology||Mouse - 14/15 amino acid residues identical; human - 11/15 amino acid residues identical.|
|Other Names||Gamma-aminobutyric acid receptor subunit rho-2, GABA(A) receptor subunit rho-2, GABA(C) receptor, Gabrr2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)RKRWTGHLETSKPSH, corresponding to amino acid residues 51-65 of rat GABA(A) ֿ2 (Accession P47742 ). Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
γ-Aminobutyric acid (GABA) is the most abundant inhibitory neurotransmitter. It is involved in roughly 40% of the inhibitory synapses1,2. GABA acts through two receptors, GABA(A) and GABA(B). To date, nineteen different GABA(A) subunits have been identified and divided in eight subunits: α (1-6), β (1-3), γ (1-3), δ, ε, ρ (1-3), θ and π. For some of the subunits, alternative splicing further increases the number of existing receptor types. They all have extracellular N- and C-termini and four transmembrane domains1. Three ρ subunits have been detected: GABA(A) ρ1, GABA(A) ρ2 and GABA(A) ρ3. Like all GABA(A) receptors the ρ subunits also assemble into a pentameric structure forming a Cl- channel. However, in contrast to all other GABA(A) subunits they mostly form homomeric entities. The GABA(A) ρ subunits display different pharmacological characteristics and were therefore once referred to GABA(C) receptors. GABA(A) and GABA(B) respectively respond to bicuculline and baclofen, whereas ρ subunits are insensitive to either drug3-7. In addition, ρ subunits also display different electrophysiological properties, and are significantly more sensitive to GABA3,5,6,8. ρ subunits are highly expressed in the retina and it was believed that they are only expressed in that area. They are however, also expressed in central and peripheral nervous systems, as well as in the gastrointestinal and cardiovascular systems1. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat GABA(A) ρ2 subunit. Anti-GABA(A) ρ2 (extracellular) antibody (#AG1273) can be used in western blot analysis and was designed to recognize GABA(A) ρ2 from human, rat and mouse samples.
References 1. Martםnez-Delgado, G. et al. (2010) Curr. Neuropharmacol. 8, 422. 2. Kumar, R.J. et al. (2008) J. Med. Chem. 51, 3825. 3. Bormann, J. (2000) Trends Pharmacol. Sci. 21, 16. 4. Bowery, N.G. (1989) Trends Pharmacol. Sci. 10, 401. 5. Johnston, G.A.R. (1996) Trends Pharmacol. Sci. 17, 319. 6. Bormann, J. and Feigenspan, A. (1995) Trends Neurosci. 18, 515. 7. Cherubini, E. and Strata, F. (1997) News Physiol. Sci. 12, 136. 8. Polenzani, L. et al. (1991) Proc. Natl. Acad. Sci. U.S.A. 88, 4318.
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