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>   home   >   Products   >   Primary Antibodies   >   GABA (A) gamma2 Receptor (extracellular) Antibody   

GABA (A) gamma2 Receptor (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - GABA (A) gamma2 Receptor (extracellular) Antibody AG1275-050
    Western blot analysis of rat brain membranes: 1. Anti-GABA(A) γ2 Receptor (extracellular) antibody (#AG1275), (1:200).  
    2. Anti-GABA(A) γ2 Receptor (extracellular) antibody, preincubated with the control peptide antigen.
  • ICC - GABA (A) gamma2 Receptor (extracellular) Antibody AG1275-050
    TNF-α-induced downregulation of surface GABA(A) γ2 Receptor Immunocytochemical staining of rat living hippocampal neurons. A. Extracellular staining of cells using Anti-GABA(A) γ2 Receptor (extracellular) antibody (#AG1275), (1:100). Representative images from time course experiments with cultured neurons undergoing TNF-α treatment ranging from 10 min to 6 h in duration. Results show a rapid downregulation in surface levels of the γ2 subunit in response to TNF-α. B. Quantification data of surface γ2 cluster area, over the 6 h time course of TNF-α treatment (n = 120 –158 images per time point; one-way ANOVA, Tukey’s post hoc test, **p < 0.01, ***p < 0.001).
    Adapted from Pribiag, H. and Stellwagen, D. (2013) with permission of the Society for Neuroscience.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P18508
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 54077 Da
Homology Human, mouse, bovine - identical; chick - 13/15 amino acid residues identical.
Additional Information
Gene ID 29709
Other Names Gamma-aminobutyric acid receptor subunit gamma-2, GABA(A) receptor subunit gamma-2, Gabrg2
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide QKSDDDYEDYASNKT(C), corresponding toֲ amino acid residues 39-53 of rat GABA(A) ־³2 Receptor (Accession number P18508). Extracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Application Details Western blot analysis (WB): - Mouse brain lysates (1:200) (see Vlachos, A. et al. (2013) in Product Citations). Live cell imaging (LCI): - Rat living hippocampal neurons (1:100) (see Pribiag, H. and Stellwagen (2013) in Product Citations). - Mouse spinal cord neurons (1:100) (see Shrivastava, A.N. in Product Citations). - Mouse hippocampal neurons (1:100) (see Chou, W.H. et al. (2010) in Product Citations). Immunocytochemistry (IC): - Rat adrenal medullary cells (1:100) (see Matsuoka, H. et al. (2008) in product Citations).
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Citations (0)

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The neurotransmitter GABA (γ-aminobutyric acid) inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells.1 There are two major types of   GABA receptors: the ionotropic GABAA (GABAA R) and the metabotropic GABAB receptors.   GABAA R belongs to the ligand gated ion channel superfamily.1,2  It is a heteropentamer, with all of its five subunits contributing to the pore formation. To date, eight subunit isoforms were cloned: α, β, γ, δ, ε, π, θ, and ρ.1 The native GABAA receptor, in most cases, consists of 2α, 2β and 1γ subunit. Three γ subunits genes have been identified in mammals.  The binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance, resulting in inhibition of neural activity.2,3  Recently, a genetic linkage between familial epilepsy syndrome and mutations in the γ2 subunit of the GABAA receptor have been demonstrated.4,5


References 1. Mihic, S.J. et al. (1994) J.Neurotransmit. Rev. 21, 127. 2. Whiting, P.J. et al. (1999) Neurochem. Int. 34, 379.  3. Ashcroft, F.M. (2000) Ion Channels and Disease Ed 1. 4. Wallace, R.H. et al. (2001) Nat. Genet. 28, 49. 5. Baulac, S. et al. (2001) Nat. Genet. 28, 46.

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$ 367.00
$ 475.00
Cat# AG1275-050
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