|Application ||WB, LCI|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||54077 Da|
|Homology||Human, mouse, bovine - identical; chick - 13/15 amino acid residues identical.|
|Other Names||Gamma-aminobutyric acid receptor subunit gamma-2, GABA(A) receptor subunit gamma-2, Gabrg2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide QKSDDDYEDYASNKT(C), corresponding toֲ amino acid residues 39-53 of rat GABA(A) ־³2 Receptor (Accession number P18508). Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Application Details||Western blot analysis (WB): - Mouse brain lysates (1:200) (see Vlachos, A. et al. (2013) in Product Citations). Live cell imaging (LCI): - Rat living hippocampal neurons (1:100) (see Pribiag, H. and Stellwagen (2013) in Product Citations). - Mouse spinal cord neurons (1:100) (see Shrivastava, A.N. in Product Citations). - Mouse hippocampal neurons (1:100) (see Chou, W.H. et al. (2010) in Product Citations). Immunocytochemistry (IC): - Rat adrenal medullary cells (1:100) (see Matsuoka, H. et al. (2008) in product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
The neurotransmitter GABA (γ-aminobutyric acid) inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells.1 There are two major types of GABA receptors: the ionotropic GABAA (GABAA R) and the metabotropic GABAB receptors. GABAA R belongs to the ligand gated ion channel superfamily.1,2 It is a heteropentamer, with all of its five subunits contributing to the pore formation. To date, eight subunit isoforms were cloned: α, β, γ, δ, ε, π, θ, and ρ.1 The native GABAA receptor, in most cases, consists of 2α, 2β and 1γ subunit. Three γ subunits genes have been identified in mammals. The binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance, resulting in inhibition of neural activity.2,3 Recently, a genetic linkage between familial epilepsy syndrome and mutations in the γ2 subunit of the GABAA receptor have been demonstrated.4,5
References 1. Mihic, S.J. et al. (1994) J.Neurotransmit. Rev. 21, 127. 2. Whiting, P.J. et al. (1999) Neurochem. Int. 34, 379. 3. Ashcroft, F.M. (2000) Ion Channels and Disease Ed 1. 4. Wallace, R.H. et al. (2001) Nat. Genet. 28, 49. 5. Baulac, S. et al. (2001) Nat. Genet. 28, 46.
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